protein

Recombinant human GOT2 protein

ATGP3143

4 ug

30 USD

Recombinant human GOT2 protein

30-430aa

ATGP3143

NP_002071.2

GOT2, also known as glutamate oxaloacetate transaminase 2, belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. glutamate oxaloacetate transaminas is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and inner-membrane mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology. Recombinant human GOT2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Aspartate aminotransferase 2 mitochondrial, KAT4, KATIV, mitAAT

0.5mg/ml (determined by Bradford assay)

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT, 0.15M NaCl

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Guidetti P, Amori L, et al. (2007). J Neurochem. 102(1):103-11.;; Lain B, Iriarte A, et al. (1995). J Biol Chem. 270(42):24732-9.

> 95% by SDS-PAGE

Specific activity is > 60unit/mg, and is defined as the amount of enzyme that convert 1umole of alpha-ketoglutarate to L-Glutamate per minute at pH 8.0 at 25C.

SDS-PAGE, Enzyme Activity

Human

Metabolism

47 kDa (424aa) confirmed by MALDI-TOF

< 1 EU per 1ug of protein (determined by LAL method)

Specific activity is > 60 units/mg, and is defined as the amount of enzyme that convert 1umole of alpha-ketoglutarate to L-Glutamate per minute at pH 8.0 at 25C.

SSWWTHV EMGPPDPILG VTEAFKRDTN SKKMNLGVGA YRDDNGKPYV LPSVRKAEAQ IAAKNLDKEY LPIGGLAEFC KASAELALGE NSEVLKSGRF VTVQTISGTG ALRIGASFLQ RFFKFSRDVF LPKPTWGNHT PIFRDAGMQL QGYRYYDPKT CGFDFTGAVE DISKIPEQSV LLLHACAHNP TGVDPRPEQW KEIATVVKKR NLFAFFDMAY QGFASGDGDK DAWAVRHFIE QGINVCLCQS YAKNMGLYGE RVGAFTMVCK DADEAKRVES QLKILIRPMY SNPPLNGARI AAAILNTPDL RKQWLQEVKV MADRIIGMRT QLVSNLKKEG STHNWQHITD QIGMFCFTGL KPEQVERLIK EFSIYMTKDG RISVAGVTSS NVGYLAHAIH QVTK

His-Tag

E.coli

Recombinant Protein

P00505

GOT2, Aspartate aminotransferase 2 mitochondrial, KAT4, KATIV, mitAAT,ATGP3143-10ug,ATGP3143-20ug,ATGP3143-50ug,ATGP3143-100ug,ATGP3143-250ug,ATGP3143-500ug,ATGP3143-1mg,ATGP3143-10,ATGP3143-20,ATGP3143-50,ATGP3143-100,ATGP3143-250,ATGP3143-500,ATGP3143-1

Yoshida T, et al. A covalent small molecule inhibitor of glutamate-oxaloacetate transaminase 1 impairs pancreatic cancer growth. (Biochem Biophys Res Commun. 2020){https://pubmed.ncbi.nlm.nih.gov/31787239/}