Recombinant human GRP58/PDIA3 protein | NKMAX ATGP3137 product information

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant human GRP58/PDIA3 protein

catalog :

ATGP3137

quantity :

10 ug

price :

60 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant human GRP58/PDIA3 protein

Domain :

25-505aa

Catalog number (SKU) :

ATGP3137

NCBI :

NP_005304

Description :

PDIA3, also known as protein disulfide-isomerase A3, is a protein of the endoplasmic reticulum that interacts with lectin chaperones calreticulin and calnexin to modulate folding of newly synthesized glycoproteins. This protein has protein disulfide isomerase activity. PDIA3 is also part of the major histocompatibility complex (MHC) class I peptide-loading complex (TAP1), which is essential for formation of the final antigen conformation and export from the endoplasmic reticulum to the cell surface. Recombinant human PDIA3 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Alternative Names :

Protein disulfide isomerase family A member 3, Glucose regulated protein 58kDa, GPR58, Protein disulfide isomerase-associated 3, 58 kDa microsomal protein, P58, ERp61, Endoplasmic reticulum resident protein 57, ER protein 57, ERp57, Disulfide isomerase ER-60, Endoplasmic reticulum resident protein 60, ER protein 60, ERp60, GRP57, PI-PLC, HsT17083

Concentration :

1mg/ml (determined by Bradford assay)

Formulation :

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT,0.1 M NaCl, and 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Vigneron N., et al. (2009) Eur J Immunol. 39(9):2371-6. ;; Forster ML., et al. (2009) J Biol Chem. 284(19):13045-56.

Purity :

> 95% by SDS-PAGE

Bioactivity Comment Only :

Specific activity > 10 A650/cm/min/mg. Enzymatic activity was confirmed by measuring the aggregation of insulin in the presence of DTT.

Applications :

SDS-PAGE, Enzyme Activity

Antigen Species :

Human

Gene Category :

Heat Shock Proteins

Molecular Weight :

58.5 kDa (518aa) confirmed by MALDI-TOF

Bioactivity Comment and Pics :

Specific activity > 20 A650/cm/min/mg. Enzymatic activity was confirmed by measuring the aggregation of insulin in the presence of DTT.

Amino Acid Sequence :

MSDV LELTDDNFES RISDTGSAGL MLVEFFAPWC GHCKRLAPEY EAAATRLKGI VPLAKVDCTA NTNTCNKYGV SGYPTLKIFR DGEEAGAYDG PRTADGIVSH LKKQAGPASV PLRTEEEFKK FISDKDASIV GFFDDSFSEA HSEFLKAASN LRDNYRFAHT NVESLVNEYD DNGEGIILFR PSHLTNKFED KTVAYTEQKM TSGKIKKFIQ ENIFGICPHM TEDNKDLIQG KDLLIAYYDV DYEKNAKGSN YWRNRVMMVA KKFLDAGHKL NFAVASRKTF SHELSDFGLE STAGEIPVVA IRTAKGEKFV MQEEFSRDGK ALERFLQDYF DGNLKRYLKS EPIPESNDGP VKVVVAENFD EIVNNENKDV LIEFYAPWCG HCKNLEPKYK ELGEKLSKDP NIVIAKMDAT ANDVPSPYEV RGFPTIYFSP ANKKLNPKKY EGGRELSDFI SYLQREATNP PVIQEEKPKK KKKAQEDL

Tag :

His-Tag

Expression System :

E.coli

Product Type Name :

Recombinant Protein

UniProt No. :

P30101

Internal Search Terms :

ERp57, PDIA3, Protein disulfide-isomerase A3, ER60, ERp57, ERp60, ERp61, GRP57, GRP58, HsT17083, P58, PI-PLC,ATGP3137-10ug,ATGP3137-20ug,ATGP3137-50ug,ATGP3137-100ug,ATGP3137-250ug,ATGP3137-500ug,ATGP3137-1mg,ATGP3137-10,ATGP3137-20,ATGP3137-50,ATGP3137-100,ATGP3137-250,ATGP3137-500,ATGP3137-1

more info or order :

NKMAX product webpage