Recombinant human P4HB protein | NKMAX ATGP3136 product information

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant human P4HB protein

catalog :

ATGP3136

quantity :

4 ug

price :

30 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant human P4HB protein

Domain :

18-508aa

Catalog number (SKU) :

ATGP3136

NCBI :

NP_000909

Description :

P4HB, prolyl 4 hydroxylase subunit beta, is a multifunctional and highly abundant enzyme that belongs to the protein disulfide isomerase family. When present as a tetramer consisting of two alpha and two beta subunits, this enzyme is involved in hydroxylation of prolyl residues in preprocollagen. This protein is also a disulfide isomerase containing two thioredoxin domains that catalyze the formation, breakage and rearrangement of disulfide bonds. Recombinant human P4HB protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Alternative Names :

Proly 4-hydroxylase subunit beta, P4Hbeta, PDI, PDIA1, PHD, PO4DB, PO4HB; ERBA2L, Protein disulfide-isomerase, Cellular thyroid hormone-binding protein, p55

Concentration :

1mg/ml (determined by Bradford assay)

Formulation :

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Pfander D., et al. (2006) Am J Pathol. 169(2):491-502.;; Kivirikko KI., et al. (1991) J Hepatol. 13 Suppl 3:S2-7.

Purity :

> 90% by SDS-PAGE

Bioactivity Comment Only :

Specific activity > 100 A650/cm/min/mg. Enzymatic activity was confirmed by measuring the aggregation of insulin in the presence of DTT.

Applications :

SDS-PAGE, Enzyme Activity

Antigen Species :

Human

Gene Category :

Heat Shock Proteins

Molecular Weight :

57.5 kDa (521aa) confirmed by MALDI-TOF

Bioactivity Comment and Pics :

Specific activity > 100 A650/cm/min/mg. Enzymatic activity was confirmed by measuring the aggregation of insulin in the presence of DTT.

Amino Acid Sequence :

MGSSHHHHHH SSGLVPRGSH MDAPEEEDHV LVLRKSNFAE ALAAHKYLLV EFYAPWCGHC KALAPEYAKA AGKLKAEGSE IRLAKVDATE ESDLAQQYGV RGYPTIKFFR NGDTASPKEY TAGREADDIV NWLKKRTGPA ATTLPDGAAA ESLVESSEVA VIGFFKDVES DSAKQFLQAA EAIDDIPFGI TSNSDVFSKY QLDKDGVVLF KKFDEGRNNF EGEVTKENLL DFIKHNQLPL VIEFTEQTAP KIFGGEIKTH ILLFLPKSVS DYDGKLSNFK TAAESFKGKI LFIFIDSDHT DNQRILEFFG LKKEECPAVR LITLEEEMTK YKPESEELTA ERITEFCHRF LEGKIKPHLM SQELPEDWDK QPVKVLVGKN FEDVAFDEKK NVFVEFYAPW CGHCKQLAPI WDKLGETYKD HENIVIAKMD STANEVEAVK VHSFPTLKFF PASADRTVID YNGERTLDGF KKFLESGGQD GAGDDDDLED LEEAEEPDME EDDDQKAVKD EL

Tag :

His-Tag

Expression System :

E.coli

Product Type Name :

Recombinant Protein

UniProt No. :

P07237

Internal Search Terms :

P4HB, Proly 4-hydroxylase subunit beta, P4Hbeta, PDI, PDIA1, PHD, PO4DB, PO4HB; ERBA2L,ATGP3136-10ug,ATGP3136-20ug,ATGP3136-50ug,ATGP3136-100ug,ATGP3136-250ug,ATGP3136-500ug,ATGP3136-1mg,ATGP3136-10,ATGP3136-20,ATGP3136-50,ATGP3136-100,ATGP3136-250,ATGP3136-500,ATGP3136-1

more info or order :

NKMAX product webpage