Recombinant e.coli gldA protein | NKMAX ATGP3130 product information

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant e.coli gldA protein

catalog :

ATGP3130

quantity :

4 ug

price :

30 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant e.coli gldA protein

Domain :

1-367aa

Catalog number (SKU) :

ATGP3130

NCBI :

NP_418380

Description :

gldA catalyzes the NAD-dependent oxidation of glycerol to dihydroxyacetone (glycerone). This protein allows microorganisms to utilize glycerol as a source of carbon under anaerobic conditions. In E. coli, an important role of GldA is also likely to regulate the intracellular level of dihydroxyacetone by catalyzing the reverse reaction, i. e. the conversion of dihydroxyacetone into glycerol. gldA possesses a broad substrate specificity, since it is also able to oxidize 1, 2-propanediol and to reduce glycolaldehyde, methylglyoxal and hydroxyacetone into ethylene glycol, lactaldehyde and 1, 2-propanediol, respectively. Recombinant E. coli gldA protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Alternative Names :

Glycerol dehydrogenase, ECK3937, JW5556

Concentration :

1mg/ml (determined by Bradford assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Subedi K.P., et al. (2008) FEMS Microbiol. Lett. 279:180-187;; Gonzalez R., et al. (2008) Metab. Eng. 10:234-245

Purity :

> 95% by SDS-PAGE

Bioactivity Comment Only :

Specific activity: > 14 Units/ml One unit will oxidize 1.0 umole of glycerol to dihydroxyacetone per minute at pH 8.0 at 25C

Applications :

Enzyme Activity,SDS-PAGE

Antigen Species :

E.coli

Gene Category :

Signal Transduction

Molecular Weight :

41.1 kDa (390aa) confirmed by MALDI-TOF

Bioactivity Comment and Pics :

Specific activity > 14 Units/ml One unit will oxidize 1.0 umole of glycerol to dihydroxyacetone per minute at pH 8.0 at 25C

Amino Acid Sequence :

MGSSHHHHHH SSGLVPRGSH MGSMDRIIQS PGKYIQGADV INRLGEYLKP LAERWLVVGD KFVLGFAQST VEKSFKDAGL VVEIAPFGGE CSQNEIDRLR GIAETAQCGA ILGIGGGKTL DTAKALAHFM GVPVAIAPTI ASTDAPCSAL SVIYTDEGEF DRYLLLPNNP NMVIVDTKIV AGAPARLLAA GIGDALATWF EARACSRSGA TTMAGGKCTQ AALALAELCY NTLLEEGEKA MLAAEQHVVT PALERVIEAN TYLSGVGFES GGLAAAHAVH NGLTAIPDAH HYYHGEKVAF GTLTQLVLEN APVEEIETVA ALSHAVGLPI TLAQLDIKED VPAKMRIVAE AACAEGETIH NMPGGATPDQ VYAALLVADQ YGQRFLQEWE

Tag :

His-Tag

Expression System :

E.coli

Product Type Name :

Recombinant Protein

UniProt No. :

P0A9S5

Internal Search Terms :

gldA, Glycerol dehydrogenase, ECK3937, JW5556,ATGP3130-10ug,ATGP3130-20ug,ATGP3130-50ug,ATGP3130-100ug,ATGP3130-250ug,ATGP3130-500ug,ATGP3130-1mg,ATGP3130-10,ATGP3130-20,ATGP3130-50,ATGP3130-100,ATGP3130-250,ATGP3130-500,ATGP3130-1

more info or order :

NKMAX product webpage