protein

Recombinant human Carbonic Anhydrase 12/CA12 protein

ATGP3124

10 ug

80 USD

Recombinant human Carbonic Anhydrase 12/CA12 protein

25-301aa

ATGP3124

NP_001209.1

CA12, also known as Carbonic anhydrase 12, is an enzyme that in humans is encoded by the CA12 gene. Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. This gene product is a type I membrane protein that is highly expressed in normal tissues, such as kidney, colon and pancreas, and has been found to be overexpressed in 10% of clear cell renal carcinomas. Recombinant human CA12, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Carbonic anhydrase 12 isoform 1, CAXII, HsT18816, Carbonate dehydratase XII, CA-XII, tumor antigen HOM-RCC-3.1.3, Carbonic Anhydrase XII

0.5mg/ml (determined by absorbance at 280nm)

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Tureci O. et al. (1998) Proc Natl Acad Sci U S A. 95(13):7608-13.;; Kyllonen MS. et al. (2003) J Histochem Cytochem. 51(9):1217-24.

> 90% by SDS-PAGE

Specific activity is > 300pmol/min/ug, and is defined as the amount of enzyme that hydrolyze 1.0pmole of 4-nitrophenyl acetate to 4-nitrophenol per minute at pH 7.5 at 37C.

SDS-PAGE, Enzyme Activity

Human

Cancer

31.94 kDa (283aa)

< 1 EU per 1ug of protein (determined by LAL method)

Specific activity is > 300 pmol/min/ug, and is defined as the amount of enzyme that hydrolyze 1.0 pmole of 4-nitrophenyl acetate to 4-nitrophenol per minute at pH 7.5 at 37C.

APVNGSKWTY FGPDGENSWS KKYPSCGGLL QSPIDLHSDI LQYDASLTPL EFQGYNLSAN KQFLLTNNGH SVKLNLPSDM HIQGLQSRYS ATQLHLHWGN PNDPHGSEHT VSGQHFAAEL HIVHYNSDLY PDASTASNKS EGLAVLAVLI EMGSFNPSYD KIFSHLQHVK YKGQEAFVPG FNIEELLPER TAEYYRYRGS LTTPPCNPTV LWTVFRNPVQ ISQEQLLALE TALYCTHMDD PSPREMINNF RQVQKFDERL VYTSFSQVQV CTAAGLS

His-Tag

Baculovirus

Recombinant Protein

O43570

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