protein

Recombinant human Akt1 protein

ATGP3118

10 ug

80 USD

Recombinant human Akt1 protein

1-480aa

ATGP3118

NP_001014432

AKT1, also known as RAC-alpha serine/threonine-protein kinase, is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Recombinant human AKT1, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Protein kinase B, Protein kinase B alpha, Proto-oncogene c-Akt, RAC-PK-alpha

0.5mg/ml (determined by absorbance at 280nm)

Liquid in. Phosphate-Buffered Saline (pH 7.4) 20% glycerol

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Lindhurst MJ, et al (2011) N Engl J Med. 365(7):611-9.

> 90% by SDS-PAGE

SDS-PAGE

Human

Signal Transduction

56.7 kDa (488aa)

< 1 EU per 1ug of protein (determined by LAL method)

MSDVAIVKEG WLHKRGEYIK TWRPRYFLLK NDGTFIGYKE RPQDVDQREA PLNNFSVAQC QLMKTERPRP NTFIIRCLQW TTVIERTFHV ETPEEREEWT TAIQTVADGL KKQEEEEMDF RSGSPSDNSG AEEMEVSLAK PKHRVTMNEF EYLKLLGKGT FGKVILVKEK ATGRYYAMKI LKKEVIVAKD EVAHTLTENR VLQNSRHPFL TALKYSFQTH DRLCFVMEYA NGGELFFHLS RERVFSEDRA RFYGAEIVSA LDYLHSEKNV VYRDLKLENL MLDKDGHIKI TDFGLCKEGI KDGATMKTFC GTPEYLAPEV LEDNDYGRAV DWWGLGVVMY EMMCGRLPFY NQDHEKLFEL ILMEEIRFPR TLGPEAKSLL SGLLKKDPKQ RLGGGSEDAK EIMQHRFFAG IVWQHVYEKK LSPPFKPQVT SETDTRYFDE EFTAQMITIT PPDQDDSMEC VDSERRPHFP QFSYSASGTA

His-Tag

Baculovirus

Recombinant Protein

P31749

Akt1,ATGP3118-10ug,ATGP3118-20ug,ATGP3118-50ug,ATGP3118-100ug,ATGP3118-250ug,ATGP3118-500ug,ATGP3118-1mg,ATGP3118-10,ATGP3118-20,ATGP3118-50,ATGP3118-100,ATGP3118-250,ATGP3118-500,ATGP3118-1

Wang Q, et al. Tumor suppressor Pdcd4 attenuates Sin1 translation to inhibit invasion in colon carcinoma. (Oncogene. 2017){https://pubmed.ncbi.nlm.nih.gov/28692058/}