protein

Recombinant human MIF protein

ATGP3101

20 ug

80 USD

Recombinant human MIF protein

1-115aa

ATGP3101

NP_002406

MIF also known as Macrophage migration inhibitory factor. MIF has been identified to be secreted by the pituitary gland and the monocyte/macrophage and to play an important role in endotoxic shock. MIF has the unique property of being released from macrophages and T cells in response to physiological concentrations of glucocorticoids. The secretion of MIF is tightly regulated and decreases at high, anti-inflammatory steroid concentration. Recombinant human MIF, fused to GST-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Macrophage migration inhibitory factor, GIF, GLIF, MMIF

1mg/ml (determined by Bradford assay)

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Bernhagen J., et al (1994) Biochemistry. 33: 14144-55.;; Bucala R., et al. (1996) FASEB J 10: 1607-13.

> 90% by SDS-PAGE

SDS-PAGE

Human

Hormones & Cytokines

39.2 kDa (345aa) confirmed by MALDI-TOF

MPMFIVNTNV PRASVPDGFL SELTQQLAQA TGKPPQYIAV HVVPDQLMAF GGSSEPCALC SLHSIGKIGG AQNRSYSKLL CGLLAERLRI SPDRVYINYY DMNAANVGWN NSTFA

GST-Tag

E.coli

Recombinant Protein

P14174

MIF,ATGP3101-10ug,ATGP3101-20ug,ATGP3101-50ug,ATGP3101-100ug,ATGP3101-250ug,ATGP3101-500ug,ATGP3101-1mg,ATGP3101-10,ATGP3101-20,ATGP3101-50,ATGP3101-100,ATGP3101-250,ATGP3101-500,ATGP3101-1

Garai J, et al. Tetralone derivatives are MIF tautomerase inhibitors and attenuate macrophage activation and amplify the hypothermic response in endotoxemic mice. (J Enzyme Inhib Med Chem. 2021){https://pubmed.ncbi.nlm.nih.gov/34225560/}