product summary
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company name :
NKMAX
product type :
protein
product name :
Recombinant mouse AST1/GOT1 protein
catalog :
ATGP3085
quantity :
20 ug
price :
80 USD
more info or order :
NKMAX product webpage
product information
Product Type :
Proteins
Item Name :
Recombinant mouse AST1/GOT1 protein
Domain :
1-413aa
Catalog number (SKU) :
ATGP3085
NCBI :
AAH02057
Description :
Got1 also known as Glutamate oxaloacetate transaminase 1 is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and mitochondrial forms, GOT1 and GOT2, respectively. GOT1 plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology. Recombinant mouse Got1 fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Alternative Names :
Transaminase A, Glutamate oxaloacetate transaminase 1 soluble, Glutamate oxaloacetate transaminase 1, Cytoplasmic, Cysteine transaminase, Cysteine aminotransferase, cCAT, cAspAT
Concentration :
0.5mg/ml (determined by Bradford assay)
Formulation :
Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol, 1mM DTT
Storage :
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
General References :
Ballif B.A., et al. (2008) J. Proteome Res. 7:311-318
Purity :
> 95% by SDS-PAGE
Bioactivity Comment Only :
Specific activity is > 80unit/mg, and is defined as the amount of enzyme that converts 1.0 micromole of alpha-ketoglutarate to L-Glutamate per minute at pH 8.0 at 25C.
Applications :
SDS-PAGE, Enzyme Activity
Antigen Species :
Mouse
Gene Category :
Metabolism
Molecular Weight :
48.6 kDa (436aa) confirmed by MALDI-TOF
Bioactivity Comment and Pics :
Specific activity is > 80 units/mg, and is defined as the amount of enzyme that converts 1.0 micromole of alpha-ketoglutarate to L-Glutamate per minute at pH 8.0 at 25C.
Amino Acid Sequence :
MAPPSVF AQVPQAPPVL VFKLTADFRD DPDPRKVNLG VGAYRTDESQ PWVLPVVRKV EQKIANDNSL NHEYLPILGL AEFRSCASRL VLGDNSPAIR ENRVGGVQSL GGTGALRIGA DFLGRWYNGT DNKNTPIYVS SPTWENHNAV FSAAGFKDIR PYCYWDAEKR GLDLQGFLND LENAPEFSIF VLHACAHNPT GTDPTPEQWK QIAAVMQRRF LFPFFDSAYQ GFASGDLEKD AWAIRYFVSE GFELFCAQSF SKNFGLYNER VGNLTVVGKE SDSVLRVLSQ MEKIVRITWS NPPAQGARIV AATLSDPELF KEWKGNVKTM ADRILTMRSE LRARLEALKT PGTWSHITEQ IGMFSFTGLN PKQVEYLVNE KHIYLLPSGR INMCGLTTKN LDYVATSIHE AVTKIQ
Tag :
His-Tag
Expression System :
E.coli
Product Type Name :
Recombinant Protein
UniProt No. :
P05201
Internal Search Terms :
Got1, Glutamate oxaloacetate transaminase 1 soluble, cAspAT,ATGP3085-10ug,ATGP3085-20ug,ATGP3085-50ug,ATGP3085-100ug,ATGP3085-250ug,ATGP3085-500ug,ATGP3085-1mg,ATGP3085-10,ATGP3085-20,ATGP3085-50,ATGP3085-100,ATGP3085-250,ATGP3085-500,ATGP3085-1
more info or order :
NKMAX product webpage
company information
NKMAX
SNUH Health Care Innovation Park 6F
172 Dolma-ro, Bundang-gu
Seongnam-si, Gyeonggi-do
https://www.nkmaxbio.com
82-31-8017-8114
headquarters: South Korea
NKMAX Co. Ltd. is a biotech company that manufactures and supplies a unique line of quality proteins and antibodies worldwide to both researchers and retailers.