Recombinant human IL-17A protein | NKMAX ATGP3083 product information

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant human IL-17A protein

catalog :

ATGP3083

quantity :

10 ug

price :

80 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant human IL-17A protein

Domain :

24-155aa

Catalog number (SKU) :

ATGP3083

NCBI :

NP_002181.1

Description :

IL17A, also known as Interleukin 17, is a cytokine that acts as a potent mediator in delayed-type reactions by increasing chemokine production in various tissues to recruit monocytes and neutrophils to the site of inflammation. It is produced by T-helper cells and is induced by IL23 which results in destructive tissue damage in delayed-type reactions. This protein is a 155 amino acid protein that is a disulfide-linked, homodimeric, secreted glycoprotein with a molecular mass of 35kDa. The structure of IL17A consists of a signal peptide of 23 amino acids followed by a 123 aa chain region characteristic of the IL17 family. An N-linked glycosylation site on the protein was first identified after purification of the protein revealed two bands, one at 15kDa and another at 20kDa. Recombinant human IL17A, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Alternative Names :

Interleukin 17A, IL-17, IL-17A, IL17, CTLA8, Cytotoxic T-lymphocyte-associated protein 8, Cytotoxic T-lymphocyte-associated serine esterase 8

Concentration :

0.25mg/ml (determined by Bradford assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 20% glycerol, 1mM EDTA, 0.1mM PMSF

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Yao Z, et al (1995) J Immunol. 155(12):5483-6.

Purity :

> 90% by SDS-PAGE

Bioactivity Comment Only :

The activity is determined by the IL-6 ELISA in a using NIH/3T3 mouse embryonic fibroblast cells. The ED50 range ≤ 7 ng/ml.

Applications :

SDS-PAGE, Bioactivity

Antigen Species :

Human

Gene Category :

Hormones & Cytokines

Molecular Weight :

15.9 kDa (138aa)

Endotoxin :

< 1 EU per 1ug of protein (determined by LAL method)

Bioactivity Comment and Pics :

Human IL-17A stimulates IL-6 secretion of the NIH/3T3 mouse embryonic fibroblast cells. The ED50 range ≤ 7 ng/ml.

Amino Acid Sequence :

GITIPRNPGC PNSEDKNFPR TVMVNLNIHN RNTNTNPKRS SDYYNRSTSP WNLHRNEDPE RYPSVIWEAK CRHLGCINAD GNVDYHMNSV PIQQEILVLR REPPHCPNSF RLEKILVSVG CTCVTPIVHH VA

Tag :

His-Tag

Expression System :

Baculovirus

Predicted nterm :

Gly24

Product Type Name :

Recombinant Protein

UniProt No. :

Q16552

Internal Search Terms :

IL17A, Interleukin 17A, CTLA8, IL-17, IL-17A, IL17,ATGP3083-10ug,ATGP3083-20ug,ATGP3083-50ug,ATGP3083-100ug,ATGP3083-250ug,ATGP3083-500ug,ATGP3083-1mg,ATGP3083-10,ATGP3083-20,ATGP3083-50,ATGP3083-100,ATGP3083-250,ATGP3083-500,ATGP3083-1

more info or order :

NKMAX product webpage