protein

Recombinant humanCarbonic Anhydrase 13/CA13 protein

ATGP3059

10 ug

80 USD

Recombinant humanCarbonic Anhydrase 13/CA13 protein

1-262aa

ATGP3059

NP_940986.1

CA13 also known as carbonic anhydrase 13 belongs to the alpha-carbonic anhydrase family. The carbonic anhydrase from a family of enzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and protons, a reversible reaction that occurs relatively slowly in the absence of catalyst. The active site of most carbonic anhydrases contains a zinc ion; they are claasified as metalloenzymes. There are at least five distinct CA families (alpha, beta, gamma, delta, and epsilon). These families have no significant amino acid sequence similarity and in most cases are thought to be an example of convergent evolution. The alpha-CAs are found in humans. Recombinant human CA13, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Carbonic anhydrase 13, CAXIII, Carbonic anhydrase XIII, Carbonate dehydratase XIII

0.5mg/ml (determined by Bradford assay)

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol, 1mM DTT

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Lamesch P., et al. (2007) Genomics 89 (3), 307-315

> 90% by SDS-PAGE

Specific activity is > 2,500pmol/min/ug, and is defined as the amount of enzyme that hydrolyze 1.0pmole of 4-nitrophenyl acetate to 4-nitrophenol per minute at pH 7.5 at 37C.

SDS-PAGE, Enzyme Activity

Human

Enzymes & Proteases

31.8 kDa (285aa) confirmed by MALDI-TOF

Specific activity is > 2,500 pmol/min/ug, and is defined as the amount of enzyme that hydrolyze 1.0 pmole of 4-nitrophenyl acetate to 4-nitrophenol per minute at pH 7.5 at 37C.

MSRLSWG YREHNGPIHW KEFFPIADGD QQSPIEIKTK EVKYDSSLRP LSIKYDPSSA KIISNSGHSF NVDFDDTENK SVLRGGPLTG SYRLRQVHLH WGSADDHGSE HIVDGVSYAA ELHVVHWNSD KYPSFVEAAH EPDGLAVLGV FLQIGEPNSQ LQKITDTLDS IKEKGKQTRF TNFDLLSLLP PSWDYWTYPG SLTVPPLLES VTWIVLKQPI NISSQQLAKF RSLLCTAEGE AAAFLVSNHR PPQPLKGRKV RASFH

His-Tag

E.coli

Recombinant Protein

Q8N1Q1

CAXIII, CA 13, CA XIII, CA13, Carbonate dehydratase 13, Carbonate dehydratase XIII, Carbonate dehydratase13, Carbonate dehydrataseXIII, Carbonic anhydrase 13, Carbonic anhydrase XIII, Carbonic anhydrase13, Carbonic anhydraseXIII, ATGP3059-10ug, ATGP3059-20ug, ATGP3059-50ug, ATGP3059-100ug, ATGP3059-250ug, ATGP3059-500ug, ATGP3059-1mg, ATGP3059-010, ATGP3059-020, ATGP3059-050, ATGP3059-100, ATGP3059-250, ATGP3059-500, ATGP3059-01M