Recombinant human ATP6V1F protein | NKMAX ATGP2947 product information

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant human ATP6V1F protein

catalog :

ATGP2947

quantity :

20 ug

price :

80 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant human ATP6V1F protein

Domain :

1-119aa

Catalog number (SKU) :

ATGP2947

NCBI :

NP_004222

Description :

ATP6V1F is a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c', and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. Recombinant human ATP6V1F, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Alternative Names :

V-type proton ATPase subunit F, V-type proton ATPase subunit F, ATP6S14, VATF, Vma7

Concentration :

0.5mg/ml (determined by Bradford assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing, 50% glycerol, 1mM DTT

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Supino R., et al. (2009) Ann. N. Y. Acad. Sci. 1171, 606-616;; Smith AN., et al. (2008) J. Bioenerg. Biomembr. 40 (4), 371-380;;

Purity :

> 90% by SDS-PAGE

Applications :

SDS-PAGE

Antigen Species :

Human

Gene Category :

Metabolism

Molecular Weight :

15.8 kDa (142aa) confirmed by MALDI-TOF

Amino Acid Sequence :

MGSSHHHHHH SSGLVPRGSH MGSMAGRGKL IAVIGDEDTV TGFLLGGIGE LNKNRHPNFL VVEKDTTINE IEDTFRQFLN RDDIGIILIN QYIAEMVRHA LDAHQQSIPA VLEIPSKEHP YDAAKDSILR RARGMFTAED LR

Tag :

His-Tag

Expression System :

E.coli

Product Type Name :

Recombinant Protein

UniProt No. :

Q16864

Internal Search Terms :

ATP6V1F, V-type proton ATPase subunit F, V-type proton ATPase subunit F, ATP6S14, VATF, Vma7,ATGP2947-10ug,ATGP2947-20ug,ATGP2947-50ug,ATGP2947-100ug,ATGP2947-250ug,ATGP2947-500ug,ATGP2947-1mg,ATGP2947-10,ATGP2947-20,ATGP2947-50,ATGP2947-100,ATGP2947-250,ATGP2947-500,ATGP2947-1

more info or order :

NKMAX product webpage