Recombinant mouse IL-33 protein | NKMAX ATGP2860 product information

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant mouse IL-33 protein

catalog :

ATGP2860

quantity :

10 ug

price :

105 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant mouse IL-33 protein

Domain :

109-266aa

Catalog number (SKU) :

ATGP2860

NCBI :

NP_001158196.1

Description :

Interleukin 33 (IL-33) is a 32kDa proinflammatory cytokine that may also regulate gene transcription in producer cells. IL-33 is structurally related to IL-1, which induces helper T cells to produce type 2 cytokines and acts through the receptor IL1RL-1 (IL1 receptor-like-1), which is known also as ST2. Binding of IL-33 to this receptor activates NF-kappa-B and MAP kinases and induces in vitro Th2 cells to produce cytokines. In vivo, IL-33 induces expression of IL-4, IL-5, IL-13 and leads to severe pathological changes in mucosal organs and in vitro, it can be divided to N-terminal fragment of 12kDa and C-terminal fragment of 18kDa by cleavage of caspase-1. Recombinant mouse IL33 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Alternative Names :

Interleukin-33, IL-33, Il1f11, NF-HEV, 230117N10Rik

Concentration :

0.5mg/ml (determined by Bradford assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol, 1mM DTT

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Schmitz J., et al (2005) Immunity 23:479-490;; Ali S., et al (2011) J. Immunol. 187:1609-1616

Purity :

> 95% by SDS-PAGE

Bioactivity Comment Only :

Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells. The ED50 range ≤ 0.1ng/ml.

Applications :

SDS-PAGE, Bioactivity

Antigen Species :

Mouse

Gene Category :

Hormones & Cytokines

Molecular Weight :

18.1 kDa (181aa) confirmed by MALDI-TOF

Endotoxin :

< 1 EU per 1ug of protein (determined by LAL method)

Bioactivity Comment and Pics :

Mouse IL-33 stimulates cell proliferation D10.G4.1 mouse helper T cells. The ED50 range ≤ 0.1ng/ml.

Amino Acid Sequence :

SIQGTSL LTQSPASLST YNDQSVSFVL ENGCYVINVD DSGKDQEQDQ VLLRYYESPC PASQSGDGVD GKKLMVNMSP IKDTDIWLHA NDKDYSVELQ RGDVSPPEQA FFVLHKKSSD FVSFECKNLP GTYIGVKDNQ LALVEEKDES CNNIMFKLSK I

Tag :

His-Tag

Expression System :

E.coli

Product Type Name :

Recombinant Protein

UniProt No. :

Q8BVZ5

Internal Search Terms :

Interleukin-33, 230117N10Rik, IL-33, Il-33, Il1f11, Interleukin 33, NF-HEV, ATGP2860-10ug, ATGP2860-20ug, ATGP2860-50ug, ATGP2860-100ug, ATGP2860-250ug, ATGP2860-500ug, ATGP2860-1mg, ATGP2860-010, ATGP2860-020, ATGP2860-050, ATGP2860-100, ATGP2860-250, ATGP2860-500, ATGP2860-01M

more info or order :

NKMAX product webpage