protein

Recombinant human IL-12 p70 protein

ATGP2843

10 ug

80 USD

Recombinant human IL-12 p70 protein

23-328aa(p40)/23-219aa(p35)

ATGP2843

NP_002178(p40)AAK84425(p35)

IL12 is a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. It is a disulfide-linked heterodimer composed of the 40 kD cytokine receptor like subunit and a 35 kD subunit. This cytokine is expressed by activated macrophages that serve as an essential inducer of Th1 cells development. IL12 has been found to be important for sustaining a sufficient number of memory/effector Th1 cells to mediate long-term protection to an intracellular pathogen. Recombinant human IL12 protein, fused to His-tag at C-terminus, was expressed in insect cells using baculovirus expression system and purified by using conventional chromatography techniques.

IL12A, IL12B, CLMF p35, CLMF p40, Cytotoxic lymphocyte maturation factor 35 kDa subunit, Cytotoxic lymphocyte maturation factor 40 kDa subunit, Interleukin 12 alpha chain, Interleukin-12 beta chain, NK cell stimulatory factor chain 1, p35, NK cell stimulatory factor chain 2, p40, NKSF1, NKSF2

0.5mg/ml (determined by absorbance at 280nm)

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Barton J.L., et al. (2000) Eur. J. Immunol. 30:3299-3308;; Bazan J.F., et al. (2001) J. Immunol. 167:1440-1446

> 90% by SDS-PAGE

The activity is determined by the IFN-g ELISA in a using NK-92 human natural killer cells. The ED50 range ≤1ng/ml.

SDS-PAGE, Bioactivity

Human

Hormones & Cytokines

34.6 kDa (p40, 306aa);;23.3 kDa (p35, 203aa) ;;The heterodimeric IL12 has a predicted Molecular weight of 57.9 kDa (509aa). Molecular weight on SDS-PAGE will appear higher.

< 1 EU per 1ug of protein (determined by LAL method)

Human IL12 stimulates IFN-g secretion of the NK-92 human natural killer cells. The ED50 range ≤1 ng/ml.

IL12B(p40);;IWELKKDVYV VELDWYPDAP GEMVVLTCDT PEEDGITWTL DQSSEVLGSG KTLTIQVKEF GDAGQYTCHK GGEVLSHSLL LLHKKEDGIW STDILKDQKE PKNKTFLRCE AKNYSGRFTC WWLTTISTDL TFSVKSSRGS SDPQGVTCGA ATLSAERVRG DNKEYEYSVE CQEDSACPAA EESLPIEVMV DAVHKLKYEN YTSSFFIRDI IKPDPPKNLQ LKPLKNSRQV EVSWEYPDTW STPHSYFSLT FCVQVQGKSK REKKDRVFTD KTSATVICRK NASISVRAQD RYYSSSWSEW ASVPCS;;IL12A(p35);;RNLPVATPDP GMFPCLHHSQ NLLRAVSNML QKARQTLEFY PCTSEEIDHE DITKDKTSTV EACLPLELTK NESCLNSRET SFITNGSCLA SRKTSFMMAL CLSSIYEDLK MYQVEFKTMN AKLLMDPKRQ IFLDQNMLAV IDELMQALNF NSETVPQKSS LEEPDFYKTK IKLCILLHAF RIRAVTIDRV MSYLNAS

His-Tag

Baculovirus

Met1

Recombinant Protein

P29460(p40)/P29459(p35)

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Kim HR, et al. Anti-cancer activity and mechanistic features of a NK cell activating molecule. (Cancer Immunol Immunother. 2009){https://pubmed.ncbi.nlm.nih.gov/19259669/}