Recombinant human RNGTT protein | NKMAX ATGP2805 product information

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant human RNGTT protein

catalog :

ATGP2805

quantity :

10 ug

price :

80 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant human RNGTT protein

Domain :

1-597aa

Catalog number (SKU) :

ATGP2805

NCBI :

NP_003791

Description :

RNGTT is a bifunctional mRNA-capping enzyme exhibiting RNA 5'-triphosphatase activity in the N-terminal part and mRNA guanylyltransferase activity in the C-terminal part. It catalyzes the first two steps of cap formation: by removing the gamma-phosphate from the 5'-triphosphate end of nascent mRNA to yield a diphosphate end, and by transferring the gmp moiety of GTP to the 5'-diphosphate terminus. Recombinant human RNGTT protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Alternative Names :

mRNA-capping enzyme, RNA guanylyltransferase and 5'-phosphatase, CAP1A, hCAP, HCE, HCE1

Concentration :

0.25mg/ml (determined by Bradford assay)

Formulation :

Liquid in. 20mM Tris-HCl buffer (pH 7.5) containing 0.2M NaCl, 40% glycerol, 2mM DTT, 0.1mM PMSF

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Yue Z., Maldonado E et al.(1997) Proc. Natl. Acad. Sci. u.S.A. 94:12898-12903.

Purity :

> 85% by SDS-PAGE

Applications :

SDS-PAGE

Antigen Species :

Human

Gene Category :

Epigenomics (Transcription & Translation)

Molecular Weight :

70.9 kDa (620aa)

Amino Acid Sequence :

MAHNKIP PRWLNCPRRG QPVAGRFLPL KTMLGPRYDS QVAEENRFHP SMLSNYLKSL KVKMGLLVDL TNTSRFYDRN DIEKEGIKYI KLQCKGHGEC PTTENTETFI RLCERFNERN PPELIGVHCT HGFNRTGFLI CAFLVEKMDW SIEAAVATFA QARPPGIYKG DYLKELFRRY GDIEEAPPPP LLPDWCFEDD EDEDEDEDGK KESEPGSSAS FGKRRKERLK LGAIFLEGVT VKGVTQVTTQ PKLGEVQQKC HQFCGWEGSG FPGAQPVSMD KQNIKLLDLK PYKVSWKADG TRYMMLIDGT NEVFMIDRDN SVFHVSNLEF PFRKDLRMHL SNTLLDGEMI IDRVNGQAVP RYLIYDIIKF NSQPVGDCDF NVRLQCIERE IISPRHEKMK TGLIDKTQEP FSVRNKPFFD ICTSRKLLEG NFAKEVSHEM DGLIFQPTGK YKPGRCDDIL KWKPPSLNSV DFRLKITRMG GEGLLPQNVG LLYVGGYERP FAQIKVTKEL KQYDNKIIEC KFENNSWVFM RQRTDKSFPN AYNTAMAVCN SISNPVTKEM LFEFIDRCTA ASQGQKRKHH LDPDTELMPP PPPKRPRPLT

Tag :

His-Tag

Expression System :

E.coli

Product Type Name :

Recombinant Protein

UniProt No. :

O60942

Internal Search Terms :

RNGTT, mRNA-capping enzyme, RNA guanylyltransferase and 5'-phosphatase, CAP1A, hCAP, HCE, HCE1,ATGP2805-10ug,ATGP2805-20ug,ATGP2805-50ug,ATGP2805-100ug,ATGP2805-250ug,ATGP2805-500ug,ATGP2805-1mg,ATGP2805-10,ATGP2805-20,ATGP2805-50,ATGP2805-100,ATGP2805-250,ATGP2805-500,ATGP2805-1

more info or order :

NKMAX product webpage