Recombinant human RNA polymerase III subunit RPC10/POLR3K protein

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant human RNA polymerase III subunit RPC10/POLR3K protein

catalog :

ATGP2759

quantity :

4 ug

price :

80 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant human RNA polymerase III subunit RPC10/POLR3K protein

Domain :

1-108aa

Catalog number (SKU) :

ATGP2759

NCBI :

NP_057394.1

Description :

PORL3K is a small essential subunit of RNA polymerase III, the polymerase responsible for synthesizing transfer and small ribosomal RNAs in eukaryotes. The carboxy-terminal domain of this subunit shares a high degree of sequence similarity to the carboxy-terminal domain of an RNA polymerase II elongation factor. This similarity in sequence is supported by functional studies showing that this subunit is required for proper pausing and termination during transcription. Pseudogenes of this gene are found on chromosomes 13 and 17 Recombinant human PORL3K protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Alternative Names :

DNA-directed RNA polymerase III subunit RPC10, C11, C11-RNP3, hRPC11, My010, RPC10, RPC11, RPC12.5

Concentration :

0.25mg/ml (determined by Bradford assay)

Formulation :

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 50% glycerol, 2mM DTT, 2mM EDTA

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Johnson,S.S., et al. (2007) Mol. Cell 26 (3), 367-379;; De Gobbi,M., et al. (2006) Science 312 (5777), 1215-1217;;

Purity :

> 85% by SDS-PAGE

Applications :

SDS-PAGE

Antigen Species :

Human

Gene Category :

Epigenomics (Transcription & Translation)

Molecular Weight :

14.7 kDa (131aa)

Amino Acid Sequence :

MLLFCPG CGNGLIVEEG QRCHRFACNT CPYVHNITRK VTNRKYPKLK EVDDVLGGAA AWENVDSTAE SCPKCEHPRA YFMQLQTRSA DEPMTTFYKC CNAQCGHRWR D

Tag :

His-Tag

Expression System :

E.coli

Product Type Name :

Recombinant Protein

UniProt No. :

Q9Y2Y1

Internal Search Terms :

POLR3K, DNA-directed RNA polymerase III subunit RPC10, C11, C11-RNP3, hRPC11, My010, RPC10, RPC11, RPC12.5,ATGP2759-10ug,ATGP2759-20ug,ATGP2759-50ug,ATGP2759-100ug,ATGP2759-250ug,ATGP2759-500ug,ATGP2759-1mg,ATGP2759-10,ATGP2759-20,ATGP2759-50,ATGP2759-100,ATGP2759-250,ATGP2759-500,ATGP2759-1

more info or order :

NKMAX product webpage