Recombinant human RTCA protein | NKMAX ATGP2735 product information

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant human RTCA protein

catalog :

ATGP2735

quantity :

4 ug

price :

80 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant human RTCA protein

Domain :

1-366aa

Catalog number (SKU) :

ATGP2735

NCBI :

NP_003720.1

Description :

RTCA catalyzes the conversion of 3'-phosphate to a 2', 3'-cyclic phosphodiester at the end of RNA. The mechanism of action of the enzyme occurs in 3 steps: (A) adenylation of the enzyme by ATP; (B) transfer of adenylate to an RNA-N3'P to produce RNA-N3'PP5'A; (C) and attack of the adjacent 2'-hydroxyl on the 3'-phosphorus in the diester linkage to produce the cyclic end product. The biological role of this enzyme is unknown but it is likely to function in some aspects of cellular RNA processing. Recombinant human RTCA protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Alternative Names :

RNA 3-terminal phosphate cyclase isoform b, RNA 3'-terminal phosphate cyclase, RPC, RTC1, RTCD1

Concentration :

0.25mg/ml (determined by Bradford assay)

Formulation :

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 20% glycerol, 1mM DTT

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Genschik P., Billy E., et al. (1997) EMBO J. 16:2955-2967

Purity :

> 90% by SDS-PAGE

Applications :

SDS-PAGE

Antigen Species :

Human

Gene Category :

Epigenomics (Transcription & Translation)

Molecular Weight :

41.7 kDa (389aa) confirmed by MALDI-TOF

Amino Acid Sequence :

MAGPRVE VDGSIMEGGG QILRVSTALS CLLGLPLRVQ KIRAGRSTPG LRPQHLSGLE MIRDLCDGQL EGAEIGSTEI TFTPEKIKGG IHTADTKTAG SVCLLMQVSM PCVLFAASPS ELHLKGGTNA EMAPQIDYTV MVFKPIVEKF GFIFNCDIKT RGYYPKGGGE VIVRMSPVKQ LNPINLTERG CVTKIYGRAF VAGVLPFKVA KDMAAAAVRC IRKEIRDLYV NIQPVQEPKD QAFGNGNGII IIAETSTGCL FAGSSLGKRG VNADKVGIEA AEMLLANLRH GGTVDEYLQD QLIVFMALAN GVSRIKTGPV TLHTQTAIHF AEQIAKAKFI VKKSEDEEDA AKDTYIIECQ GIGMTNPNL

Tag :

His-Tag

Expression System :

E.coli

Product Type Name :

Recombinant Protein

UniProt No. :

O00442

Internal Search Terms :

RTCA, RNA 3-terminal phosphate cyclase isoform b, RNA 3'-terminal phosphate cyclase, RPC, RTC1, RTCD1,ATGP2735-10ug,ATGP2735-20ug,ATGP2735-50ug,ATGP2735-100ug,ATGP2735-250ug,ATGP2735-500ug,ATGP2735-1mg,ATGP2735-10,ATGP2735-20,ATGP2735-50,ATGP2735-100,ATGP2735-250,ATGP2735-500,ATGP2735-1

more info or order :

NKMAX product webpage