protein

Recombinant human RBM17 protein

ATGP2451

10 ug

80 USD

Recombinant human RBM17 protein

1-401aa

ATGP2451

NP_116294.1

RNA binding motif protein 17, also known as RBM17, is splice factor that binds to the single stranded 3'AG at the exon/intron border and promotes its utilization in the second catalytic step. This protein is involved in the regulation of alternative splicing and the utilization of cryptic splice sites. This protein promotes the utilization of a cryptic splice site created by the beta-110 mutation in the HBB gene. The resulting frameshift leads to sickle cell anemia. Recombinant human RBM17 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

RNA binding motif protein 17, SPF45

0.5mg/ml (determined by Bradford assay)

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Lallena M.J., et al. (2002) Cell. 109:285-296;; Carrascal M., et al. (2008) J. Proteome Res. 7:5167-5176

> 90% by SDS-PAGE

SDS-PAGE

Human

Epigenomics (Transcription & Translation)

47.1 kDa (421aa) confirmed by MALDI-TOF

MSLYDDLGVE TSDSKTEGWS KNFKLLQSQL QVKKAALTQA KSQRTKQSTV LAPVIDLKRG GSSDDRQIVD TPPHVAAGLK DPVPSGFSAG EVLIPLADEY DPMFPNDYEK VVKRQREERQ RQRELERQKE IEEREKRRKD RHEASGFARR PDPDSDEDED YERERRKRSM GGAAIAPPTS LVEKDKELPR DFPYEEDSRP RSQSSKAAIP PPVYEEQDRP RSPTGPSNSF LANMGGTVAH KIMQKYGFRE GQGLGKHEQG LSTALSVEKT SKRGGKIIVG DATEKDASKK SDSNPLTEIL KCPTKVVLLR NMVGAGEVDE DLEVETKEEC EKYGKVGKCV IFEIPGAPDD EAVRIFLEFE RVESAIKAVV DLNGRYFGGR VVKACFYNLD KFRVLDLAEQ V

His-Tag

E.coli

Recombinant Protein

Q96I25

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