Recombinant human PCBP1 protein | NKMAX ATGP2314 product information

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant human PCBP1 protein

catalog :

ATGP2314

quantity :

20 ug

price :

80 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant human PCBP1 protein

Domain :

1-163aa

Catalog number (SKU) :

ATGP2314

NCBI :

NP_006187

Description :

PCBP1 appears to be multifunctional. It along with PCBP-2 and hnRNPK corresponds to the major cellular poly (rC) -binding protein. It contains three K-homologous (KH) domains which may be involved in RNA binding. This protein together with PCBP-2 also functions as translational coactivators of poliovirus RNA via a sequence-specific interaction with stem-loop IV of the IRES and promotes poliovirus RNA replication by binding to its 5'-terminal cloverleaf structure. It has also been implicated in translational control of the 15-lipoxygenase mRNA, human Papillomavirus type 16 L2 mRNA, and hepatitis A virus RNA. PCBP1 is also suggested to play a part in formation of a sequence-specific alpha-globin mRNP complex which is associated with alpha-globin mRNA stability. Recombinant human PCBP1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Alternative Names :

Poly(rC) binding protein 1, Poly(rC) binding protein 1, hnRNP-E1, hnRNP-X, HNRPE1, HNRPX

Concentration :

1mg/ml (determined by Bradford assay)

Formulation :

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 20% glycerol, 1mM DTT

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Beausoleil S.A., et al. (2006) Nat. Biotechnol. 24:1285-1292;; Yu L.-R., et al. (2007) J. Proteome Res. 6:4150-4162;;

Purity :

> 85% by SDS-PAGE

Applications :

SDS-PAGE

Antigen Species :

Human

Gene Category :

Epigenomics (Transcription & Translation)

Molecular Weight :

19.6 kDa (186aa) confirmed by MALDI-TOF

Amino Acid Sequence :

MGSSHHHHHH SSGLVPRGSH MGSMDAGVTE SGLNVTLTIR LLMHGKEVGS IIGKKGESVK RIREESGARI NISEGNCPER IITLTGPTNA IFKAFAMIID KLEEDINSSM TNSTAASRPP VTLRLVVPAT QCGSLIGKGG CKIKEIREST GAQVQVAGDM LPNSTERAIT IAGVPQSVTE CVKQIC

Tag :

His-Tag

Expression System :

E.coli

Product Type Name :

Recombinant Protein

UniProt No. :

Q15365

Internal Search Terms :

PCBP1, Poly(rC) binding protein 1, Poly(rC) binding protein 1, hnRNP-E1, hnRNP-X, HNRPE1, HNRPX,ATGP2314-10ug,ATGP2314-20ug,ATGP2314-50ug,ATGP2314-100ug,ATGP2314-250ug,ATGP2314-500ug,ATGP2314-1mg,ATGP2314-10,ATGP2314-20,ATGP2314-50,ATGP2314-100,ATGP2314-250,ATGP2314-500,ATGP2314-1

more info or order :

NKMAX product webpage