protein

Recombinant human Kallikrein 2/KLK2 protein

ATGP2175

20 ug

80 USD

Recombinant human Kallikrein 2/KLK2 protein

25-261aa

ATGP2175

NP_005542

KLK2 is a member of the grandular kallikrein protein family. Kallikreins are a subgroup of serine proteases that are clustered on chromosome 19. Members of this family are involved in a diverse array of biological functions. The protein is a highly active trypsin-like serine protease that selectively cleaves at arginine residues. KLK2 is primarily expressed in prostatic tissue and is responsible for cleaving pro-prostate-specific antigen into its enzymatically active form. This gene is highly expressed in prostate tumor cells and may be a prognostic maker for prostate cancer risk. Alternate splicing results in both coding and non-coding transcript variants. Recombinant human KLK2 protein, fused to His-tag at N-terminus, was expressed in E. coli.

Kallikrein related peptidase 2, Glandular kallikrein-1, hGK-1, Tissue kallikrein-2, Prostatic kallikrein 2

1mg/ml (determined by Bradford assay)

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 0.4M urea, 10% glycerol

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Cargill M., et al. (1999) Nat. Genet. 22:231-238;; Gan L., et al. (2000) Gene. 257:119-130

> 85% by SDS-PAGE

SDS-PAGE, Denatured

Human

Cancer

28.5 kDa (260aa)

MGSSHHHHHH SSGLVPRGSH MGSIVGGWEC EKHSQPWQVA VYSHGWAHCG GVLVHPQWVL TAAHCLKKNS QVWLGRHNLF EPEDTGQRVP VSHSFPHPLY NMSLLKHQSL RPDEDSSHDL MLLRLSEPAK ITDVVKVLGL PTQEPALGTT CYASGWGSIE PEEFLRPRSL QCVSLHLLSN DMCARAYSEK VTEFMLCAGL WTGGKDTCGG DSGGPLVCNG VLQGITSWGP EPCALPEKPA VYTKVVHYRK WIKDTIAANP

His-Tag

E.coli

Recombinant Protein

P20151

Kallikrein2, Kallikrein-related peptidase 2, hK2, KLK2A2,ATGP2175-10ug,ATGP2175-20ug,ATGP2175-50ug,ATGP2175-100ug,ATGP2175-250ug,ATGP2175-500ug,ATGP2175-1mg,ATGP2175-10,ATGP2175-20,ATGP2175-50,ATGP2175-100,ATGP2175-250,ATGP2175-500,ATGP2175-1

Lee SW, et al. Simple and robust antibody microarray-based immunoassay platform for sensitive and selective detection of PSA and hK2 toward accurate diagnosis of prostate cancer. (Sensing and Bio-Sensing Research, 2015){https://doi.org/10.1016/j.sbsr.2015.01.003.};;Lee SW, et al. A Highly Sensitive Porous Silicon (P-Si)-Based Human Kallikrein 2 (hK2) Immunoassay Platform toward Accurate Diagnosis of Prostate Cancer. (Sensors (Basel). 2015){https://www.ncbi.nlm.nih.gov/pubmed/26007739};;Lee SW, et al. Porous silicon microarray for simultaneous fluorometric immunoassay of the biomarkers prostate-specific antigen and human glandular kallikrein 2. (Microchim Acta. 2016){https://doi.org/10.1007/s00604-016-1986-1}