Recombinant e.coli mug protein | NKMAX ATGP2057 product information

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant e.coli mug protein

catalog :

ATGP2057

quantity :

10 ug

price :

80 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant e.coli mug protein

Domain :

1-168aa

Catalog number (SKU) :

ATGP2057

NCBI :

NP_417540

Description :

G/u mismatch-specific DNA glycosylase, xanthine DNA glycosylase, also known as mug, belongs to the TDG/mug DNA glycosylase family. It has been proposed that the Mug protein excises 3, N4-ethenocytosine and removes the uracil base from mismatches in the order of u:G>u:A, although the biological role remains unclear. The enzyme uracil-N-Glycosylase removes uracil from the DNA leaving an AP site. It is capable of hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of the DNA and the mispaired base. The complementary strand guanine functions in substrate recognition. Recombinant E. coli mug protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Alternative Names :

G/u mismatch-specific DNA glycosylase, xanthine DNA glycosylase, dug, ECK3058, JW3040, ygjF

Concentration :

0.5mg/ml (determined by Bradford assay)

Formulation :

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 20% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Lee HW., et al. (2010) J Biol Chem. 285(53):41483-90;; Gallinari P., et al. (1996) Nature. 383(6602):735-8.;;

Purity :

> 90% by SDS-PAGE

Applications :

SDS-PAGE

Antigen Species :

E.coli

Gene Category :

Enzymes & Proteases

Molecular Weight :

21.1 kDa (191aa) confirmed by MALDI-TOF

Amino Acid Sequence :

MGSSHHHHHH SSGLVPRGSH MGSMVEDILA PGLRVVFCGI NPGLSSAGTG FPFAHPANRF WKVIYQAGFT DRQLKPQEAQ HLLDYRCGVT KLVDRPTVQA NEVSKQELHA GGRKLIEKIE DYQPQALAIL GKQAYEQGFS QRGAQWGKQT LTIGSTQIWV LPNPSGLSRV SLEKLVEAYR ELDQALVVRG R

Tag :

His-Tag

Expression System :

E.coli

Product Type Name :

Recombinant Protein

UniProt No. :

P0A9H1

Internal Search Terms :

mug, G/u mismatch-specific DNA glycosylase, xanthine DNA glycosylase, dug, ECK3058, JW3040, ygjF,ATGP2057-10ug,ATGP2057-20ug,ATGP2057-50ug,ATGP2057-100ug,ATGP2057-250ug,ATGP2057-500ug,ATGP2057-1mg,ATGP2057-10,ATGP2057-20,ATGP2057-50,ATGP2057-100,ATGP2057-250,ATGP2057-500,ATGP2057-1

more info or order :

NKMAX product webpage