Recombinant e.coli mutY protein | NKMAX ATGP2056 product information

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant e.coli mutY protein

catalog :

ATGP2056

quantity :

10 ug

price :

140 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant e.coli mutY protein

Domain :

1-350aa

Catalog number (SKU) :

ATGP2056

NCBI :

NP_417436

Description :

Adenine DNA glycosylase, also known as mutY, is an adenine DNA glycosylase active on DNA substrates containing A/G, A/8-oxoG, or A/C mismatches and also has a weak guanine glycosylase activity on G/8-oxoG-containing DNA. mutY is crucial for the avoidance of mutations resulting from oxidative DNA damage. Increasing levels of mutY in A549 cells exposed to oxygen and infrared radiation leads to improvements in cell survival. It is abundant in neurons where mitochondrial genomes exposed to reactive oxygen species (ROS) that damage DNA must maintain integrity over the entire mammalian life span. Recombinant E. coil mutY protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Alternative Names :

Adenine DNA glycosylase, ECK2956, JW2928, mica, mutB

Concentration :

0.25mg/ml (determined by Bradford assay)

Formulation :

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 20% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Lee H M., et al. (2004) J Neurochem. 88:394-400;; Tao H., et al. (2004) Carcinogenesis. 25:1859-1866.;;

Purity :

> 90% by SDS-PAGE

Applications :

SDS-PAGE

Antigen Species :

E.coli

Gene Category :

Enzymes & Proteases

Molecular Weight :

41.5 kDa (373aa) confirmed by MALDI-TOF

Amino Acid Sequence :

MGSSHHHHHH SSGLVPRGSH MGSMQASQFS AQVLDWYDKY GRKTLPWQID KTPYKVWLSE VMLQQTQVAT VIPYFERFMA RFPTVTDLAN APLDEVLHLW TGLGYYARAR NLHKAAQQVA TLHGGKFPET FEEVAALPGV GRSTAGAILS LSLGKHFPIL DGNVKRVLAR CYAVSGWPGK KEVENKLWSL SEQVTPAVGV ERFNQAMMDL GAMICTRSKP KCSLCPLQNG CIAAANNSWA LYPGKKPKQT LPERTGYFLL LQHEDEVLLA QRPPSGLWGG LYCFPQFADE ESLRQWLAQR QIAADNLTQL TAFRHTFSHF HLDIVPMWLP VSSFTGCMDE GNALWYNLAQ PPSVGLAAPV ERLLQQLRTG APV

Tag :

His-Tag

Expression System :

E.coli

Product Type Name :

Recombinant Protein

UniProt No. :

P17802

Internal Search Terms :

mutY, Adenine DNA glycosylase, ECK2956, JW2928, mica, mutB,ATGP2056-10ug,ATGP2056-20ug,ATGP2056-50ug,ATGP2056-100ug,ATGP2056-250ug,ATGP2056-500ug,ATGP2056-1mg,ATGP2056-10,ATGP2056-20,ATGP2056-50,ATGP2056-100,ATGP2056-250,ATGP2056-500,ATGP2056-1

more info or order :

NKMAX product webpage