Recombinant e.coli phrB protein | NKMAX ATGP2044 product information

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant e.coli phrB protein

catalog :

ATGP2044

quantity :

4 ug

price :

80 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant e.coli phrB protein

Domain :

1-472aa

Catalog number (SKU) :

ATGP2044

NCBI :

NP_415236

Description :

Deoxyribodipyrimidine photolyase, also known as phrB, is involved in repair of uV radiation-induced DNA damage. This protein catalyzes the light-dependent monomerization (300-600 nm) of cyclobutyl pyrimidine dimers (in cis-syn configuration), which are formed between adjacent bases on the same DNA strand upon exposure to ultraviolet radiation. Recombinant E. coli phrB protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Alternative Names :

Deoxyribodipyrimidine photolyase, ECK0697, JW0698, phrB

Concentration :

0.25mg/ml (determined by Bradford assay)

Formulation :

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 50% glycerol, 1mM DTT

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Byrdin M., et al. (2003) Proc. Natl. Acad. Sci. u.S.A. 100:8676-8681;; Weber S., et al. (2005) Biochim. Biophys. Acta. 1707:1-23;;

Purity :

> 90% by SDS-PAGE

Applications :

SDS-PAGE

Antigen Species :

E.coli

Gene Category :

Enzymes & Proteases

Molecular Weight :

56.1 kDa (495aa)

Amino Acid Sequence :

MGSSHHHHHH SSGLVPRGSH MGSMTTHLVW FRQDLRLHDN LALAAACRNS SARVLALYIA TPRQWATHNM SPRQAELINA QLNGLQIALA EKGIPLLFRE VDDFVASVEI VKQVCAENSV THLFYNYQYE VNERARDVEV ERALRNVVCE GFDDSVILPP GAVMTGNHEM YKVFTPFKNA WLKRLREGMP ECVAAPKVRS SGSIEPSPSI TLNYPRQSFD TAHFPVEEKA AIAQLRQFCQ NGAGEYEQQR DFPAVEGTSR LSASLATGGL SPRQCLHRLL AEQPQALDGG AGSVWLNELI WREFYRHLIT YHPSLCKHRP FIAWTDRVQW QSNPAHLQAW QEGKTGYPIV DAAMRQLNST GWMHNRLRMI TASFLVKDLL IDWREGERYF MSQLIDGDLA ANNGGWQWAA STGTDAAPYF RIFNPTTQGE KFDHEGEFIR QWLPELRDVP GKVVHEPWKW AQKAGVTLDY PQPIVEHKEA RVQTLAAYEA ARKGK

Tag :

His-Tag

Expression System :

E.coli

Product Type Name :

Recombinant Protein

UniProt No. :

P00914

Internal Search Terms :

phrB, Deoxyribodipyrimidine photolyase, ECK0697, JW0698, phrB,ATGP2044-10ug,ATGP2044-20ug,ATGP2044-50ug,ATGP2044-100ug,ATGP2044-250ug,ATGP2044-500ug,ATGP2044-1mg,ATGP2044-10,ATGP2044-20,ATGP2044-50,ATGP2044-100,ATGP2044-250,ATGP2044-500,ATGP2044-1

more info or order :

NKMAX product webpage