Recombinant human CPSF4 protein | NKMAX ATGP2023 product information

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant human CPSF4 protein

catalog :

ATGP2023

quantity :

20 ug

price :

80 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant human CPSF4 protein

Domain :

1-244aa

Catalog number (SKU) :

ATGP2023

NCBI :

NP_001075028

Description :

Inhibition of the nuclear export of poly (A) -containing mRNAs caused by the influenza A virus NS1 protein requires its effector domain. The NS1 effector domain functionally interacts with the cellular 30 kDa subunit of CPSF4 an essential component of the 3' end processing machinery of cellular pre-mRNAs. In influenza virus-infected cells, the NS1 protein is physically associated with cleavage and polyadenylation specific factor 4, 30kD subunit. Binding of the NS1 protein to the 30 kDa protein in vitro prevents CPSF binding to the RNA substrate and inhibits 3' end cleavage and polyadenylation of host pre-mRNAs. Recombinant human CPSF4 protein, fused to His-tag at N-terminus, was expressed in E. coli.

Alternative Names :

cleavage and polyadenylation specific factor 430kDa, cleavage and polyadenylation specific factor 4,30kDa, CPSF30, NAR, NEB1

Concentration :

1mg/ml (determined by Bradford assay)

Formulation :

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 0.4M uREA, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Jenny,A., et al. (1994) Mol. Cell. Biol. 14 (12), 8183-8190;; Barabino,S.M., et al. (1997) Nephrol Genes Dev. 11 (13), 1703-1716;;

Purity :

> 85% by SDS-PAGE

Applications :

SDS-PAGE,Denatured

Antigen Species :

Human

Gene Category :

Epigenomics (Transcription & Translation)

Molecular Weight :

29.9 kDa (267aa)

Amino Acid Sequence :

MGSSHHHHHH SSGLVPRGSH MGSMQEIIAS VDHIKFDLEI AVEQQLGAQP LPFPGMDKSG AAVCEFFLKA ACGKGGMCPF RHISGEKTVV CKHWLRGLCK KGDQCEFLHE YDMTKMPECY FYSKFGECSN KECPFLHIDP ESKIKDCPWY DRGFCKHGPL CRHRHTRRVI CVNYLVGFCP EGPSCKFMHP RFELPMGTTE QPPLPQQTQP PAKQRTPQVI GVMQSQNSSA GNRGPRPLEQ VTCYKCGEKG HYANRCTKGH LAFLSGQ

Tag :

His-Tag

Expression System :

E.coli

Product Type Name :

Recombinant Protein

UniProt No. :

O95639

Internal Search Terms :

CPSF4, cleavage and polyadenylation specific factor 430kDa, cleavage and polyadenylation specific factor 4,30kDa, CPSF30, NAR, NEB1,ATGP2023-10ug,ATGP2023-20ug,ATGP2023-50ug,ATGP2023-100ug,ATGP2023-250ug,ATGP2023-500ug,ATGP2023-1mg,ATGP2023-10,ATGP2023-20,ATGP2023-50,ATGP2023-100,ATGP2023-250,ATGP2023-500,ATGP2023-1

more info or order :

NKMAX product webpage