Recombinant human YOD1 protein | NKMAX ATGP2019 product information

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant human YOD1 protein

catalog :

ATGP2019

quantity :

10 ug

price :

120 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant human YOD1 protein

Domain :

1-348aa

Catalog number (SKU) :

ATGP2019

NCBI :

NP_061036.3

Description :

YOD1 is a highly conserved deubiquitinating enzyme of the ovarian tumor (otubain) family, whose function has yet to be assigned in mammalian cells. YOD1 is a constituent of a multiprotein complex with p97 as its nucleus, suggesting a functional link to a pathway responsible for the dislocation of misfolded proteins from the endoplasmic reticulum. Expression of a YOD1 variant deprived of its deubiquitinating activity imposes a halt on the dislocation reaction, as judged by the stabilization of various dislocation substrates. Recombinant human YOD1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Alternative Names :

ubiquitin thioesterase OTu1, ubiquitin thioesterase OTu1, DuBA8, OTuD2, PRO0907, RP11-164O23.1

Concentration :

0.25mg/ml (determined by Bradford assay)

Formulation :

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 30% glycerol, 1mM DTT

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Ernst,R., et al. (2009) Mol. Cell 36 (1), 28-38

Purity :

> 90% by SDS-PAGE

Applications :

SDS-PAGE

Antigen Species :

Human

Gene Category :

Signal Transduction

Molecular Weight :

40.7 kDa (371aa) confirmed by MALDI-TOF

Amino Acid Sequence :

MFGPAKG RHFGVHPAPG FPGGVSQQAA GTKAGPAGAW PVGSRTDTMW RLRCKAKDGT HVLQGLSSRT RVRELQGQIA AITGIAPGGQ RILVGYPPEC LDLSNGDTIL EDLPIQSGDM LIIEEDQTRP RSSPAFTKRG ASSYVRETLP VLTRTVVPAD NSCLFTSVYY VVEGGVLNPA CAPEMRRLIA QIVASDPDFY SEAILGKTNQ EYCDWIKRDD TWGGAIEISI LSKFYQCEIC VVDTQTVRID RFGEDAGYTK RVLLIYDGIH YDPLQRNFPD PDTPPLTIFS SNDDIVLVQA LELADEARRR RQFTDVNRFT LRCMVCQKGL TGQAEAREHA KETGHTNFGE V

Tag :

His-Tag

Expression System :

E.coli

Product Type Name :

Recombinant Protein

UniProt No. :

Q5VVQ6

Internal Search Terms :

YOD1, ubiquitin thioesterase OTu1, ubiquitin thioesterase OTu1, DuBA8, OTuD2, PRO0907, RP11-164O23.1,ATGP2019-10ug,ATGP2019-20ug,ATGP2019-50ug,ATGP2019-100ug,ATGP2019-250ug,ATGP2019-500ug,ATGP2019-1mg,ATGP2019-10,ATGP2019-20,ATGP2019-50,ATGP2019-100,ATGP2019-250,ATGP2019-500,ATGP2019-1

more info or order :

NKMAX product webpage