protein

Recombinant human ADI1 protein

ATGP1975

20 ug

80 USD

Recombinant human ADI1 protein

1-179aa

ATGP1975

NP_060739

ADI1 is an enzyme that belongs to the acireductone dioxygenase family of metal-binding enzymes, which are involved in methionine salvage. This enzyme may regulate mRNA processing in the nucleus, and may carry out different functions depending on its localization. Also, it down-regulates cell migration mediated by MMP14. Recombinant human ADI1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Acireductone dioxygenase 1, APL1, ARD, FLJ10913, HMFT1638, MTCBP-1, SIPL

0.5mg/ml (determined by Bradford assay)

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Gotoh,I., et al. (2007) Genes Cells 12 (1), 105-117

> 90% by SDS-PAGE

SDS-PAGE

Human

Metabolism

25.6 kDa (216aa) confirmed by MALDI-TOF

MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSSMVL AWYMDDAPGD PRQPHRPDPG RPVGLEQLRR LGVLYWKLDA DKYENDPELE KIRRERNYSW MDIITICKDK LPNYEEKIKM FYEEHLHLDD EIRYILDGSG YFDVRDKEDQ WIRIFMEKGD MVTLPAGIYH RFTVDEKNYT KAMRLFVGEP VWTAYNRPAD HFEARGQYVK FLAQTA

His-Tag

E.coli

Recombinant Protein

Q9BV57

ADI1, Acireductone dioxygenase 1, APL1, ARD, FLJ10913, HMFT1638, MTCBP-1, SIPL,ATGP1975-10ug,ATGP1975-20ug,ATGP1975-50ug,ATGP1975-100ug,ATGP1975-250ug,ATGP1975-500ug,ATGP1975-1mg,ATGP1975-10,ATGP1975-20,ATGP1975-50,ATGP1975-100,ATGP1975-250,ATGP1975-500,ATGP1975-1

Bae DH, et al. Acireductone dioxygenase 1 (ADI1) is regulated by cellular iron by a mechanism involving the iron chaperone, PCBP1, with PCBP2 acting as a potential co-chaperone. (Biochim Biophys Acta Mol Basis Dis. 2020){https://pubmed.ncbi.nlm.nih.gov/32480040/}