Recombinant human RNA polymerase II subunit RPB11-b1/POLR2J2 protein

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant human RNA polymerase II subunit RPB11-b1/POLR2J2 protein

catalog :

ATGP1952

quantity :

10 ug

price :

105 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant human RNA polymerase II subunit RPB11-b1/POLR2J2 protein

Domain :

1-115aa

Catalog number (SKU) :

ATGP1952

NCBI :

NP_116581

Description :

POLR2J2 is a member of the RNA polymerase II subunit 11 gene family, which includes three genes in a cluster on chromosome 7q22. 1 and a pseudogene on chromosome 7p13. The founding member of family, DNA directed RNA polymerase II polypeptide J, has been shown to encode a subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This locus produces multiple, alternatively spliced transcripts that potentially express isoforms with distinct C-termini compared to DNA directed RNA polymerase II polypeptide J. Most or all variants are spliced to include additional non-coding exons at the 3' end which makes them candidates for nonsense-mediated decay (NMD). Consequently, it is not known if this locus expresses a protein or proteins in vivo. Recombinant human POLR2J2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Alternative Names :

DNA-directed RNA polymerase II subunit RPB11-b1, HRPB11B, RPB11b1

Concentration :

0.25mg/ml (determined by Bradford assay)

Formulation :

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT, 250mM Imidazole

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Shpakovskii,D.G., et al. (2004) Bioorg. Khim. 30 (6), 621-625;; Benga,W.J., et al. (2005) Nucleic Acids Res. 33 (11), 3582-3590

Purity :

> 95% by SDS-PAGE

Applications :

SDS-PAGE

Antigen Species :

Human

Gene Category :

Epigenomics (Transcription & Translation)

Molecular Weight :

15.5 kDa (138aa) confirmed by MALDI-TOF

Amino Acid Sequence :

MGSSHHHHHH SSGLVPRGSH MGSMNAPPAF ESFLLFEGEK ITINKDTKVP KACLFTINKE DHTLGNIIKS QLLKDPQVLF AGYKVPHPLE HKIIIRVQTT PDYSPQEAFT NAITDLISEL SLLEERFRTC LLPLRLLP

Tag :

His-Tag

Expression System :

E.coli

Product Type Name :

Recombinant Protein

UniProt No. :

Q9GZM3

Internal Search Terms :

POLR2J2, DNA-directed RNA polymerase II subunit RPB11-b1, HRPB11B, RPB11b1,ATGP1952-10ug,ATGP1952-20ug,ATGP1952-50ug,ATGP1952-100ug,ATGP1952-250ug,ATGP1952-500ug,ATGP1952-1mg,ATGP1952-10,ATGP1952-20,ATGP1952-50,ATGP1952-100,ATGP1952-250,ATGP1952-500,ATGP1952-1

more info or order :

NKMAX product webpage