protein

Recombinant human LCAT protein

ATGP1899

20 ug

80 USD

Recombinant human LCAT protein

25-440aa

ATGP1899

NP_000220

LCAT (lecithin-cholesterol acyltransferase), also known as phosphatidylcholine-sterol O-acyltransferase, belongs to the AB hydrolase superfamily. LCAT is responsible for the esterification of the free cholesterol of plasma lipoproteins. This enzyme is converts free cholesterol into cholesteryl ester (a more hydrophobic form of cholesterol), which is then sequestered into the core of a lipoprotein particle, eventually making the newly synthesized HDL spherical and forcing the reaction to become unidirectional since the particles are removed from the surface. It is central enzyme in the extracellular metabolism of plasma lipoproteins and required for remodeling high-density lipoprotein particles into their spherical forms. Recombinant human LCAT protein, fused to His-tag at N-terminus, was expressed in E. coli.

Phosphatidylcholine-sterol acyltransferase, lecithin-cholesterol acyltransferase, phosphatidylcholine-sterol O-acyltransferase

0.5mg/ml (determined by Bradford assay)

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 0.4M urea, 10% glycerol

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Clay M.A., et al. (2000) J. Biol. Chem. 275:9019-9025;; Karavia EA, et al. (2012) J Nutr Biochem. 2012 Jul 19.

> 85% by SDS-PAGE

SDS-PAGE, Denatured

Human

Metabolism

49.8 kDa (441aa)

MGSSHHHHHH SSGLVPRGSH MGSHMFWLLN VLFPPHTTPK AELSNHTRPV ILVPGCLGNQ LEAKLDKPDV VNWMCYRKTE DFFTIWLDLN MFLPLGVDCW IDNTRVVYNR SSGLVSNAPG VQIRVPGFGK TYSVEYLDSS KLAGYLHTLV QNLVNNGYVR DETVRAAPYD WRLEPGQQEE YYRKLAGLVE EMHAAYGKPV FLIGHSLGCL HLLYFLLRQP QAWKDRFIDG FISLGAPWGG SIKPMLVLAS GDNQGIPIMS SIKLKEEQRI TTTSPWMFPS RMAWPEDHVF ISTPSFNYTG RDFQRFFADL HFEEGWYMWL QSRDLLAGLP APGVEVYCLY GVGLPTPRTY IYDHGFPYTD PVGVLYEDGD DTVATRSTEL CGLWQGRQPQ PVHLLPLHGI QHLNMVFSNL TLEHINAILL GAYRQGPPAS PTASPEPPPP E

His-Tag

E.coli

Recombinant Protein

P04180

LCAT, Phosphatidylcholine-sterol acyltransferase, lecithin-cholesterol acyltransferase, phosphatidylcholine-sterol O-acyltransferase,ATGP1899-10ug,ATGP1899-20ug,ATGP1899-50ug,ATGP1899-100ug,ATGP1899-250ug,ATGP1899-500ug,ATGP1899-1mg,ATGP1899-10,ATGP1899-20,ATGP1899-50,ATGP1899-100,ATGP1899-250,ATGP1899-500,ATGP1899-1

Ishibashi R, et al. Immune-mediated acquired lecithin-cholesterol acyltransferase deficiency: A case report and literature review. (J Clin Lipidol. 2018){https://pubmed.ncbi.nlm.nih.gov/29937398/}