Recombinant human TYW5 protein | NKMAX ATGP1851 product information

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant human TYW5 protein

catalog :

ATGP1851

quantity :

20 ug

price :

80 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant human TYW5 protein

Domain :

1-315aa

Catalog number (SKU) :

ATGP1851

NCBI :

NP_001034782

Description :

tRNA wybutosine-synthesizing protein 5, also known as TYW5, acts as a component of the wybutosine biosynthesis pathway. Wybutosine is a hyper modified guanosine with a tricyclic base found at the 3'-position adjacent to the anticodon of eukaryotic phenylalanine tRNA. TYW5 catalyzes the hydroxylation of 7- (a-amino-a-carboxypropyl) wyosine (yW-72) into undermodified hydroxywybutosine (OHyW). OHyW is a derivative of wybutosine found in higher eukaryotes. Recombinant human TYW5 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Alternative Names :

tRNA wybutosine-synthesizing protein 5, C2orf60, Htyw5

Concentration :

1mg/ml (determined by Bradford assay)

Formulation :

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Noma A., et al. (2010) J. Biol. Chem. 285:34503-34507;; Kato M., et al. (2011) Nucleic Acids Res. 39:1576-1585;;

Purity :

> 95% by SDS-PAGE

Applications :

SDS-PAGE

Antigen Species :

Human

Gene Category :

Epigenomics (Transcription & Translation)

Molecular Weight :

38.9 kDa (338aa) confirmed by MALDI-TOF

Amino Acid Sequence :

MGSSHHHHHH SSGLVPRGSH MGSMAGQHLP VPRLEGVSRE QFMQHLYPQR KPLVLEGIDL GPCTSKWTVD YLSQVGGKKE VKIHVAAVAQ MDFISKNFVY RTLPFDQLVQ RAAEEKHKEF FVSEDEKYYL RSLGEDPRKD VADIRKQFPL LKGDIKFPEF FKEEQFFSSV FRISSPGLQL WTHYDVMDNL LIQVTGKKRV VLFSPRDAQY LYLKGTKSEV LNIDNPDLAK YPLFSKARRY ECSLEAGDVL FIPALWFHNV ISEEFGVGVN IFWKHLPSEC YDKTDTYGNK DPTAASRAAQ ILDRALKTLA ELPEEYRDFY ARRMVLHIQD KAYSKNSE

Tag :

His-Tag

Expression System :

E.coli

Product Type Name :

Recombinant Protein

UniProt No. :

A2RUC4

Internal Search Terms :

TYW5, tRNA wybutosine-synthesizing protein 5, C2orf60, Htyw5,ATGP1851-10ug,ATGP1851-20ug,ATGP1851-50ug,ATGP1851-100ug,ATGP1851-250ug,ATGP1851-500ug,ATGP1851-1mg,ATGP1851-10,ATGP1851-20,ATGP1851-50,ATGP1851-100,ATGP1851-250,ATGP1851-500,ATGP1851-1

more info or order :

NKMAX product webpage