Recombinant human RNA polymerases I, II, and III subunit RPABC1/POLR2E protein

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant human RNA polymerases I, II, and III subunit RPABC1/POLR2E protein

catalog :

ATGP1843

quantity :

20 ug

price :

80 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant human RNA polymerases I, II, and III subunit RPABC1/POLR2E protein

Domain :

1-210aa

Catalog number (SKU) :

ATGP1843

NCBI :

NP_002686

Description :

POLR2E, also as known as DNA-directed RNA polymerases I, II, and III subunit RPABC1, belongs to the archaeal RpoH/eukaryotic RPB5 RNA polymerase subunit family. POLR2E is the fifth largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit is shared by the other two DNA-directed RNA polymerases and is present in two-fold molar excess over the other polymerase subunits. POLR2E is a DNA-dependent RNA polymerase that catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Recombinant human POLR2E protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Alternative Names :

RNA polymerases I, II, and III subunit RPABC1/POLR2E protein

Concentration :

1mg/ml (determined by Bradford assay)

Formulation :

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 10% glycerol, 1mM DTT

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Kershnar E., et al. (1998) J. Biol. Chem. 273:34444-34453;; Dorjsuren D., et al. (1998) Mol. Cell. Biol. 18:7546-7555;;

Purity :

> 90% by SDS-PAGE

Applications :

SDS-PAGE

Antigen Species :

Human

Gene Category :

Epigenomics (Transcription & Translation)

Molecular Weight :

27 kDa (233aa) confirmed by MALDI-TOF

Amino Acid Sequence :

MDDEEET YRLWKIRKTI MQLCHDRGYL VTQDELDQTL EEFKAQFGDK PSEGRPRRTD LTVLVAHNDD PTDQMFVFFP EEPKVGIKTI KVYCQRMQEE NITRALIVVQ QGMTPSAKQS LVDMAPKYIL EQFLQQELLI NITEHELVPE HVVMTKEEVT ELLARYKLRE NQLPRIQAGD PVARYFGIKR GQVVKIIRPS ETAGRYITYR LVQ

Tag :

His-Tag

Expression System :

E.coli

Product Type Name :

Recombinant Protein

UniProt No. :

P19388

Internal Search Terms :

POLR2E, DNA-directed RNA polymerases I II and III subunit RPABC1, DNA-directed RNA polymerases I, II, and III subunit RPABC1, hRPB25, hsRPB5, RPABC1, RPB5, XAP4,ATGP1843-10ug,ATGP1843-20ug,ATGP1843-50ug,ATGP1843-100ug,ATGP1843-250ug,ATGP1843-500ug,ATGP1843-1mg,ATGP1843-10,ATGP1843-20,ATGP1843-50,ATGP1843-100,ATGP1843-250,ATGP1843-500,ATGP1843-1

more info or order :

NKMAX product webpage