protein

Recombinant human ACAT1 protein

ATGP1800

20 ug

80 USD

Recombinant human ACAT1 protein

34-427aa

ATGP1800

NP_000010

ACAT1, also known as acetoacetyl-CoA thiolase, is an enzyme belongs to belongs to the membrane-bound acyltransferase family and Sterol o-acyltransferase subfamily. ACAT1 is an acetyl-CoA C-acetyltransferase enzyme. This enzyme catalyzes the reversible formation of acetoacetyl-CoA from two molecules of acetyl-CoA. It plays a role in lipoprotein assembly and dietary cholesterol absorption. In addition to its acyltransferase activity, it may act as a ligase. Recombinant human ACAT1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

acetyl-CoA acetyltransferase 1, ACAT, MAT, T2, THIL

1mg/ml (determined by Bradford assay)

Liquid in. 20mM Tris-HCl buffer (pH 7.5) containing 0.1M NaCl, 10% glycerol, 1mM DTT

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Haapalainen, A.M., et al. (2007) Biochemistry 46: 4305-4321.;; Thompson, S.L. et al. (1990) J. Biol. Chem. 265:5731-5735.;;

> 95% by SDS-PAGE

SDS-PAGE

Human

Metabolism

43.8 kDa (417aa) confirmed by MALDI-TOF

VSKPTLK EVVIVSATRT PIGSFLGSLS LLPATKLGSI AIQGAIEKAG IPKEEVKEAY MGNVLQGGEG QAPTRQAVLG AGLPISTPCT TINKVCASGM KAIMMASQSL MCGHQDVMVA GGMESMSNVP YVMNRGSTPY GGVKLEDLIV KDGLTDVYNK IHMGSCAENT AKKLNIARNE QDAYAINSYT RSKAAWEAGK FGNEVIPVTV TVKGQPDVVV KEDEEYKRVD FSKVPKLKTV FQKENGTVTA ANASTLNDGA AALVLMTADA AKRLNVTPLA RIVAFADAAV EPIDFPIAPV YAASMVLKDV GLKKEDIAMW EVNEAFSLVV LANIKMLEID PQKVNINGGA VSLGHPIGMS GARIVGHLTH ALKQGEYGLA SICNGGGGAS AMLIQKL

His-Tag

E.coli

Recombinant Protein

P24752

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