Recombinant human AMPK beta 1/PRKAB1 protein | NKMAX ATGP1786 product information

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant human AMPK beta 1/PRKAB1 protein

catalog :

ATGP1786

quantity :

10 ug

price :

150 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant human AMPK beta 1/PRKAB1 protein

Domain :

1-270aa

Catalog number (SKU) :

ATGP1786

NCBI :

NP_006244.2

Description :

5'-AMP-activated protein kinase subunit beta-1, also known as PRKAB1, inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Beta non-catalytic subunit acts as a scaffold on which the AMPK complex assembles, via its C-terminus that bridges alpha (PRKAA1 or PRKAA2) and gamma subunits (PRKAG1, PRKAG2 or PRKAG3). Recombinant human PRKAB1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Alternative Names :

5'-AMP-activated protein kinase subunit beta-1, AMPK, HAMPKb, PRKAB1, Protein kinase AMP-activated non-catalytic subunit beta 1, AMPK subunit beta-1, AMPKb

Concentration :

0.5mg/ml (determined by Bradford assay)

Formulation :

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Hardie D.G., et al. (2007) Nat. Rev. Mol. Cell Biol. 8:774-785;; Towler M.C., et al. (2007) Circ. Res. 100:328-341;;

Purity :

> 85% by SDS-PAGE

Applications :

SDS-PAGE

Antigen Species :

Human

Gene Category :

Metabolism

Molecular Weight :

32.8 kDa (293aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

Amino Acid Sequence :

MGNTSSE RAALERHGGH KTPRRDSSGG TKDGDRPKIL MDSPEDADLF HSEEIKAPEK EEFLAWQHDL EVNDKAPAQA RPTVFRWTGG GKEVYLSGSF NNWSKLPLTR SHNNFVAILD LPEGEHQYKF FVDGQWTHDP SEPIVTSQLG TVNNIIQVKK TDFEVFDALM VDSQKCSDVS ELSSSPPGPY HQEPYVCKPE ERFRAPPILP PHLLQVILNK DTGISCDPAL LPEPNHVMLN HLYALSIKDG VMVLSATHRY KKKYVTTLLY KPI

Tag :

His-Tag

Expression System :

E.coli

Product Type Name :

Recombinant Protein

UniProt No. :

Q9Y478

Internal Search Terms :

AMPK b1, 5'-AMP-activated protein kinase subunit beta-1, AMPK, HAMPKb,ATGP1786-10ug,ATGP1786-20ug,ATGP1786-50ug,ATGP1786-100ug,ATGP1786-250ug,ATGP1786-500ug,ATGP1786-1mg,ATGP1786-10,ATGP1786-20,ATGP1786-50,ATGP1786-100,ATGP1786-250,ATGP1786-500,ATGP1786-1

more info or order :

NKMAX product webpage