product summary
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company name :
NKMAX
product type :
protein
product name :
Recombinant human GILT/IFI30 protein
catalog :
ATGP1753
quantity :
10 ug
price :
105 USD
more info or order :
NKMAX product webpage
product information
Product Type :
Proteins
Item Name :
Recombinant human GILT/IFI30 protein
Domain :
58-232aa
Catalog number (SKU) :
ATGP1753
NCBI :
NP_006323
Description :
IFI30, also as known as gamma-interferon-inducible lysosomal thiol reductase, belongs to the GILT family. This protein is a lysosomal thiol reductase that at low pH can reduce protein disulfide bonds. The enzyme is expressed constitutively in antigen-presenting cells and induced by gamma-interferon in other cell types. This enzyme has an important role in MHC class II-restricted antigen processing. This protein facilitates the generation of MHC class II-restricted epitodes from disulfide bond-containing antigen by the endocytic reduction of disulfide bonds. Also facilitates MHC class I-restricted recognition of exogenous antigens containing disulfide bonds by CD8+ T-cells or cross-presentation. Recombinant human IFI30 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by conventional column chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.
Alternative Names :
Gamma-interferon-inducible lysosomal thiol reductase, GILT, IFI-30, IP30
Concentration :
1mg/ml (determined by Bradford assay)
Formulation :
Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 10% glycerol,1mM DTT
Storage :
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
General References :
Arunachalam B, et al. (2000) Proc Natl Acad Sci u S A 97 (2): 745-50. ;; Luster AD, et al. (1988) J Biol Chem 263 (24): 12036-43.
Purity :
> 85% by SDS-PAGE
Applications :
SDS-PAGE
Antigen Species :
Human
Gene Category :
Immunology
Molecular Weight :
22.5 kDa (199aa) confirmed by MALDI-TOF
Amino Acid Sequence :
NAPLVN VTLYYEALCG GCRAFLIREL FPTWLLVMEI LNVTLVPYGN AQEQNVSGRW EFKCQHGEEE CKFNKVEACV LDELDMELAF LTIVCMEEFE DMERSLPLCL QLYAPGLSPD TIMECAMGDR GMQLMHANAQ RTDALQPPHE YVPWVTVNGK PLEDQTQLLT LVCQLYQGK
Tag :
His-Tag
Expression System :
E.coli
Product Type Name :
Recombinant Protein
UniProt No. :
P13284
Internal Search Terms :
GILT, IFI30,ATGP1753-10ug,ATGP1753-20ug,ATGP1753-50ug,ATGP1753-100ug,ATGP1753-250ug,ATGP1753-500ug,ATGP1753-1mg,ATGP1753-10,ATGP1753-20,ATGP1753-50,ATGP1753-100,ATGP1753-250,ATGP1753-500,ATGP1753-1
more info or order :
NKMAX product webpage
company information
NKMAX
SNUH Health Care Innovation Park 6F
172 Dolma-ro, Bundang-gu
Seongnam-si, Gyeonggi-do
https://www.nkmaxbio.com
82-31-8017-8114
headquarters: South Korea
NKMAX Co. Ltd. is a biotech company that manufactures and supplies a unique line of quality proteins and antibodies worldwide to both researchers and retailers.