protein

Recombinant human alpha-Smooth Muscle Actin/ACTA2 protein

ATGP1719

10 ug

105 USD

Recombinant human alpha-Smooth Muscle Actin/ACTA2 protein

3-377aa

ATGP1719

NP_001604.1

ACTA2 belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Defects in this gene cause aortic aneurysm familial thoracic type 6. Multiple alternatively spliced variants, encoding the same protein, have been identified. Recombinant human ACTA2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Aortic smooth muscle actin, Alpha-actin-2, Cell growth-inhibiting gene 46 protein, Actin, Aortic smooth muscle, Intermediate form, ACTSA, ACTVS, GIG46

1mg/ml (determined by Bradford assay)

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Sakai,H., et al. (2012) Hum. Genet. 131 (4), 591-599;; Regalado,E., et al. (2011) Am. J. Med. Genet. A 155A (9), 2125-2130.

> 85% by SDS-PAGE

SDS-PAGE

Human

Cardiovascular

44.4 kDa (400aa) confirmed by MALDI-TOF

EEEDS TALVCDNGSG LCKAGFAGDD APRAVFPSIV GRPRHQGVMV GMGQKDSYVG DEAQSKRGIL TLKYPIEHGI ITNWDDMEKI WHHSFYNELR VAPEEHPTLL TEAPLNPKAN REKMTQIMFE TFNVPAMYVA IQAVLSLYAS GRTTGIVLDS GDGVTHNVPI YEGYALPHAI MRLDLAGRDL TDYLMKILTE RGYSFVTTAE REIVRDIKEK LCYVALDFEN EMATAASSSS LEKSYELPDG QVITIGNERF RCPETLFQPS FIGMESAGIH ETTYNSIMKC DIDIRKDLYA NNVLSGGTTM YPGIADRMQK EITALAPSTM KIKIIAPPER KYSVWIGGSI LASLSTFQQM WISKQEYDEA GPSIVHRKCF

His-Tag

E.coli

Recombinant Protein

P62736

ACTA2, Actin aortic smooth muscle, Actin, aortic smooth muscle, AAT6, ACTSA, MYMY5,ATGP1719-10ug,ATGP1719-20ug,ATGP1719-50ug,ATGP1719-100ug,ATGP1719-250ug,ATGP1719-500ug,ATGP1719-1mg,ATGP1719-10,ATGP1719-20,ATGP1719-50,ATGP1719-100,ATGP1719-250,ATGP1719-500,ATGP1719-1

Gordon O, et al. α-actinin accounts for the bioactivity of actin preparations in inducing STAT target genes in Drosophila melanogaster. (Elife. 2018){https://pubmed.ncbi.nlm.nih.gov/30260317/}