product summary
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company name :
NKMAX
product type :
protein
product name :
Recombinant human SIAH1 protein
catalog :
ATGP1669
quantity :
10 ug
price :
105 USD
more info or order :
product information
Product Type :
Proteins
Item Name :
Recombinant human SIAH1 protein
Domain :
90-282aa
Catalog number (SKU) :
ATGP1669
NCBI :
NP_003022
Description :
E3 ubiquitin-protein ligase SIAH1, also known as SIAH1, is a member of the seven in absentia homolog (SIAH) family. SIAH1 is a tumor suppressor protein that is expressed in intestinal epithelium and activated during apoptosis. SIAH1 contains an N-terminal RING-finger domain, which is required for proteolysis, and a cystein-rich C-terminal domain, which regulates oligomerization and SIAH binding to target proteins. SIAH1 is known to cause indirect degradation of beta-catenin through formation of a complex with Siah-interacting protein (SIP), Skp1 and Ebi. Recombinant human SIAH1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Alternative Names :
E3 ubiquitin-protein ligase SIAH1, SIAH1A
Concentration :
0.25mg/ml (determined by Bradford assay)
Formulation :
Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 40% glycerol,1mM DTT
Storage :
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
General References :
Hu G., et al. (1999) Mol Cell Biol. 19: 724-732;; Matsuzawas S., et al. (1998) EMBO J. 17: 2736-2747.
Purity :
> 95% by SDS-PAGE
Applications :
SDS-PAGE
Antigen Species :
Human
Gene Category :
Ubiquitination
Molecular Weight :
24.1 kDa (216aa) confirmed by MALDI-TOF
Amino Acid Sequence :
MGSSHHHHHH SSGLVPRGSH MGSVANSVLF PCKYASSGCE ITLPHTEKAD HEELCEFRPY SCPCPGASCK WQGSLDAVMP HLMHQHKSIT TLQGEDIVFL ATDINLPGAV DWVMMQSCFG FHFMLVLEKQ EKYDGHQQFF AIVQLIGTRK QAENFAYRLE LNGHRRRLTW EATPRSIHEG IATAIMNSDC LVFDTSIAQL FAENGNLGIN VTISMC
Tag :
His-Tag
Expression System :
E.coli
Product Type Name :
Recombinant Protein
UniProt No. :
Q8IUQ4
Internal Search Terms :
SIAH1, E3 ubiquitin-protein ligase SIAH1, SIAH1A,ATGP1669-10ug,ATGP1669-20ug,ATGP1669-50ug,ATGP1669-100ug,ATGP1669-250ug,ATGP1669-500ug,ATGP1669-1mg,ATGP1669-10,ATGP1669-20,ATGP1669-50,ATGP1669-100,ATGP1669-250,ATGP1669-500,ATGP1669-1
more info or order :
company information
NKMAX
SNUH Health Care Innovation Park 6F
172 Dolma-ro, Bundang-gu
Seongnam-si, Gyeonggi-do
https://www.nkmaxbio.com172 Dolma-ro, Bundang-gu
Seongnam-si, Gyeonggi-do
82-31-8017-8114
headquarters: South Korea
NKMAX Co. Ltd. is a biotech company that manufactures and supplies a unique line of quality proteins and antibodies worldwide to both researchers and retailers.