protein

Recombinant human GCLM protein

ATGP1657

20 ug

80 USD

Recombinant human GCLM protein

1-274aa

ATGP1657

NP_002052

Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase, is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. Gamma glutamylcysteine synthetase deficiency has been implicated in some forms of hemolytic anemia. Recombinant human GCLM protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Glutamate-cysteine ligase modifier subunit, Glutamate-cysteine ligase, modifier subunit, GLCLR

1mg/ml (determined by Bradford assay)

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 0.15M NaCl

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Hanzawa,R., et al. (2011) Psychiatry Clin. Neurosci. 65 (1), 39-46

> 90% by SDS-PAGE

SDS-PAGE

Human

Metabolism

33.3 kDa (298aa) confirmed by MALDI-TOF

MGTDSR AAKALLARAR TLHLQTGNLL NWGRLRKKCP STHSEELHDC IQKTLNEWSS QINPDLVREF PDVLECTVSH AVEKINPDER EEMKVSAKLF IVESNSSSST RSAVDMACSV LGVAQLDSVI IASPPIEDGV NLSLEHLQPY WEELENLVQS KKIVAIGTSD LDKTQLEQLY QWAQVKPNSN QVNLASCCVM PPDLTAFAKQ FDIQLLTHND PKELLSEASF QEALQESIPD IQAHEWVPLW LLRYSVIVKS RGIIKSKGYI LQAKRRGS

His-Tag

E.coli

Recombinant Protein

P48507

GCLM, Glutamate-cysteine ligase modifier subunit, Glutamate-cysteine ligase, modifier subunit, GLCLR,ATGP1657-10ug,ATGP1657-20ug,ATGP1657-50ug,ATGP1657-100ug,ATGP1657-250ug,ATGP1657-500ug,ATGP1657-1mg,ATGP1657-10,ATGP1657-20,ATGP1657-50,ATGP1657-100,ATGP1657-250,ATGP1657-500,ATGP1657-1