product summary
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company name :
NKMAX
product type :
protein
product name :
Recombinant human EGLN3/PHD3 protein
catalog :
ATGP1609
quantity :
10 ug
price :
120 USD
more info or order :
product information
Product Type :
Proteins
Item Name :
Recombinant human EGLN3/PHD3 protein
Domain :
1-239aa
Catalog number (SKU) :
ATGP1609
NCBI :
NP_071356
Description :
EGLN3, a member of the EGLN family of prolyl hydroxylases, has been shown to catalyze hydroxylation of the alpha subunit of hypoxia-inducible factor-alpha, which targets hypoxia-inducible factor-alpha for ubiquitination by a ubiquitin ligase complex containing the von Hippel-Lindau (VHL) tumor suppressor. EGLN3 is the most important isozyme in limiting physiological activation of HIFs (particularly HIF2A) in hypoxia. Also hydroxylates PKM2 in hypoxia, limiting glycolysis. under normoxia, hydroxylates and regulates the stability of ADRB2. Recombinant human EGLN3 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.
Alternative Names :
Egl nine homolog 3, HIFPH3, PHD3
Concentration :
0.25mg/ml (determined by Bradford assay)
Formulation :
Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 50% glycerol, 0.3M NaCl, 5mM DTT, 2mM EDTA
Storage :
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
General References :
Epstein A.C.R., et al. (2001) Cell. 107:43-54;; Lee S., et al. (2000) Cancer Cell. 8:155-167
Purity :
> 90% by SDS-PAGE
Applications :
SDS-PAGE
Antigen Species :
Human
Gene Category :
Metabolism
Molecular Weight :
29.8 kDa (263aa) confirmed by MALDI-TOF
Amino Acid Sequence :
MPLGHI MRLDLEKIAL EYIVPCLHEV GFCYLDNFLG EVVGDCVLER VKQLHCTGAL RDGQLAGPRA GVSKRHLRGD QITWIGGNEE GCEAISFLLS LIDRLVLYCG SRLGKYYVKE RSKAMVACYP GNGTGYVRHV DNPNGDGRCI TCIYYLNKNW DAKLHGGILR IFPEGKSFIA DVEPIFDRLL FFWSDRRNPH EVQPSYATRY AMTVWYFDAE ERAEAKKKFR NLTRKTESAL TED
Tag :
His-Tag
Expression System :
E.coli
Product Type Name :
Recombinant Protein
UniProt No. :
Q9H6Z9
Internal Search Terms :
EGLN3, PHD3, Egl nine homolog 3, HIFPH3, PHD3,ATGP1609-10ug,ATGP1609-20ug,ATGP1609-50ug,ATGP1609-100ug,ATGP1609-250ug,ATGP1609-500ug,ATGP1609-1mg,ATGP1609-10,ATGP1609-20,ATGP1609-50,ATGP1609-100,ATGP1609-250,ATGP1609-500,ATGP1609-1
more info or order :
company information
NKMAX
SNUH Health Care Innovation Park 6F
172 Dolma-ro, Bundang-gu
Seongnam-si, Gyeonggi-do
https://www.nkmaxbio.com172 Dolma-ro, Bundang-gu
Seongnam-si, Gyeonggi-do
82-31-8017-8114
headquarters: South Korea
NKMAX Co. Ltd. is a biotech company that manufactures and supplies a unique line of quality proteins and antibodies worldwide to both researchers and retailers.