protein

Recombinant human G6PD protein

ATGP1533

20 ug

80 USD

Recombinant human G6PD protein

1-515aa

ATGP1533

NP_001035810.1

Glucose-6-phosphate dehydrogenase (G6PD) is the rate-limiting enzyme of the pentose phosphate pathway, a metabolic pathway that supplies reducing energy to cells by maintaining the level of NADPH. G6PD converts glucose-6-phosphate into 6-phosphoglucono-delta-lactone and simultaneously produce NADPH. The NADPH in turn maintains the level of glutathione in these cells that helps protect the red blood cells against oxidative damage. G6PD deficiency cause acute hemolytic anemia. Recombinant human G6PD protein, fused to His-tag at N-terminus, was expressed in insect cell using baculovirus expression system and purified by using conventional chromatography.

glucose-6-phosphate 1-dehydrogenase, G6PD1, G6PDH

0.5mg/ml (determined by Bradford assay)

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1mM PMSF, 2mM EDTA, 2mM DTT, 200mM NaCl

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Huang Y., et al. (2008) Mol Genet Metab. 93(1):44-53.;; Zimny A., et al. (2003) Pol Arch Med Wewn. 110(5):1327-33

> 95% by SDS-PAGE

Specific activity is > 45unit/mg, obtained by measuring the increase of beta-NADPH in absorbance at 340 nm resulting from the reduction of beta-NADP. One unit oxidizes 1.0 umole D-glucose-6-phosphate to 6-phospho-D-gluconate per min in the presence of beta-NADP at pH 7.4 at 25C.

SDS-PAGE, Enzyme Activity

Human

Enzymes & Proteases

61.4 kDa (535aa)

< 1 EU per 1ug of protein (determined by LAL method)

Specific activity is > 45 units/mg, obtained by measuring the increase of beta-NADPH in absorbance at 340 nm resulting from the reduction of beta-NADP. One unit oxidizes 1.0 umole D-glucose-6-phosphate to 6-phospho-D-gluconate per min in the presence of beta-NADP at pH 7.4 at 25C.

MAEQVALSRT QVCGILREEL FQGDAFHQSD THIFIIMGAS GDLAKKKIYP TIWWLFRDGL LPENTFIVGY ARSRLTVADI RKQSEPFFKA TPEEKLKLED FFARNSYVAG QYDDAASYQR LNSHMNALHL GSQANRLFYL ALPPTVYEAV TKNIHESCMS QIGWNRIIVE KPFGRDLQSS DRLSNHISSL FREDQIYRID HYLGKEMVQN LMVLRFANRI FGPIWNRDNI ACVILTFKEP FGTEGRGGYF DEFGIIRDVM QNHLLQMLCL VAMEKPASTN SDDVRDEKVK VLKCISEVQA NNVVLGQYVG NPDGEGEATK GYLDDPTVPR GSTTATFAAV VLYVENERWD GVPFILRCGK ALNERKAEVR LQFHDVAGDI FHQQCKRNEL VIRVQPNEAV YTKMMTKKPG MFFNPEESEL DLTYGNRYKN VKLPDAYERL ILDVFCGSQM HFVRSDELRE AWRIFTPLLH QIELEKPKPI PYIYGSRGPT EADELMKRVG FQYEGTYKWV NPHKL

His-Tag

Baculovirus

Recombinant Protein

P11413

G6PD, glucose-6-phosphate 1-dehydrogenase, G6PD1,ATGP1533-10ug,ATGP1533-20ug,ATGP1533-50ug,ATGP1533-100ug,ATGP1533-250ug,ATGP1533-500ug,ATGP1533-1mg,ATGP1533-10,ATGP1533-20,ATGP1533-50,ATGP1533-100,ATGP1533-250,ATGP1533-500,ATGP1533-1, Glucose-6-phosphate 1-dehydrogenase, zwf, Glucose-6-phosphate 1-dehydrogenase G6PD, G6pdx, Glucose 6 phosphate 1 dehydrogenase, MET19, POS10, Zwf1p

Ogasawara Y, Ishii K. Exposure to chrysotile asbestos causes carbonylation of glucose 6-phosphate dehydrogenase through a reaction with lipid peroxidation products in human lung epithelial cells. (Toxicol Lett. 2010){https://pubmed.ncbi.nlm.nih.gov/20211231/}