Recombinant human Glutathione S-transferase alpha 4/GSTA4 protein

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant human Glutathione S-transferase alpha 4/GSTA4 protein

catalog :

ATGP1504

quantity :

20 ug

price :

80 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant human Glutathione S-transferase alpha 4/GSTA4 protein

Domain :

1-222aa

Catalog number (SKU) :

ATGP1504

NCBI :

NP_001503

Description :

GSTA4, also known as glutathione S-transferase A4, belongs to the GST superfamily. This enzyme is involved in cellular defense against toxic, carcinogenic, and pharmacologically active electrophilic compounds. GSTA4 shows very high activity with reactive carbonyl compounds such as alk-2-enals. GSTA4 is highly effective in catalyzing the conjugate addition of reduced glutathione to 4-hydroxynonenal, an important product of peroxidative degradation of arachidonic acid and a commonly used biomarker for oxidative damage in tissue. This enzyme is expressed at a high level in brain, placenta, and skeletal muscle and much lower in lung and liver. Recombinant human GSTA4 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Alternative Names :

Glutathione S-transferase alpha 4, GSTA4-4, GTA4

Concentration :

1mg/ml (determined by Bradford assay)

Formulation :

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 2mM DTT, 100mM NaCl

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Bruns C.M., et al. (1999) J. Mol. Biol. 288:427-439;; Balogh L.M., et al. (2010) Biochemistry 49:1541-1548

Purity :

> 95% by SDS-PAGE

Applications :

SDS-PAGE

Antigen Species :

Human

Gene Category :

Metabolism

Molecular Weight :

28.2 kDa (246aa) confirmed by MALDI-TOF

Amino Acid Sequence :

MAARPK LHYPNGRGRM ESVRWVLAAA GVEFDEEFLE TKEQLYKLQD GNHLLFQQVP MVEIDGMKLV QTRSILHYIA DKHNLFGKNL KERTLIDMYV EGTLDLLELL IMHPFLKPDD QQKEVVNMAQ KAIIRYFPVF EKILRGHGQS FLVGNQLSLA DVILLQTILA LEEKIPNILS AFPFLQEYTV KLSNIPTIKR FLEPGSKKKP PPDEIYVRTV YNIFRP

Tag :

His-Tag

Expression System :

E.coli

Product Type Name :

Recombinant Protein

UniProt No. :

O15217

Internal Search Terms :

GSTA4, glutathione S-transferase alpha 4, GSTA4-4, GTA4,ATGP1504-10ug,ATGP1504-20ug,ATGP1504-50ug,ATGP1504-100ug,ATGP1504-250ug,ATGP1504-500ug,ATGP1504-1mg,ATGP1504-10,ATGP1504-20,ATGP1504-50,ATGP1504-100,ATGP1504-250,ATGP1504-500,ATGP1504-1

more info or order :

NKMAX product webpage