product summary
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company name :
NKMAX
product type :
protein
product name :
Recombinant e.coli pykF protein
catalog :
ATGP1503
quantity :
20 ug
price :
80 USD
more info or order :
NKMAX product webpage
product information
Product Type :
Proteins
Item Name :
Recombinant e.coli pykF protein
Domain :
1-470aa
Catalog number (SKU) :
ATGP1503
NCBI :
NP_416191
Description :
pyrF (pyruvate kinase) belongs to the pyruvate kinase family. pykF is an enzyme involved in glycolysis. It catalyzes the transfer of a phosphate group from phosphoenolpyruvate (PEP) to ADP, yielding one molecule of pyruvate and one molecule of ATP. This process also requires a Magnesium ion. This step is the final one in the glycolytic pathway, which produces pyruvate molecules, the final product of aerobic glycolysis. Recombinant E. coli pykF protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Alternative Names :
pyruvate kinase I, ECK1672, JW1666
Concentration :
1mg/ml (determined by Bradford assay)
Formulation :
Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 10% glycerol
Storage :
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
General References :
Liapounova, Na, et al. (2006) Eukaryotic cell 5 (12): 2138-46,;; Valentini G., et al. (2000) J. Biol. Chem. 275:18145-18152
Purity :
> 95% by SDS-PAGE
Applications :
SDS-PAGE
Antigen Species :
E.coli
Gene Category :
Enzymes & Proteases
Molecular Weight :
53.3 kDa (494aa)
Amino Acid Sequence :
MGSSHHHHHH SSGLVPRGSH MGSHMKKTKI VCTIGPKTES EEMLAKMLDA GMNVMRLNFS HGDYAEHGQR IQNLRNVMSK TGKTAAILLD TKGPEIRTMK LEGGNDVSLK AGQTFTFTTD KSVIGNSEMV AVTYEGFTTD LSVGNTVLVD DGLIGMEVTA IEGNKVICKV LNNGDLGENK GVNLPGVSIA LPALAEKDKQ DLIFGCEQGV DFVAASFIRK RSDVIEIREH LKAHGGENIH IISKIENQEG LNNFDEILEA SDGIMVARGD LGVEIPVEEV IFAQKMMIEK CIRARKVVIT ATQMLDSMIK NPRPTRAEAG DVANAILDGT DAVMLSGESA KGKYPLEAVS IMATICERTD RVMNSRLEFN NDNRKLRITE AVCRGAVETA EKLDAPLIVV ATQGGKSARA VRKYFPDATI LALTTNEKTA HQLVLSKGVV PQLVKEITST DDFYRLGKEL ALQSGLAHKG DVVVMVSGAL VPSGTTNTAS VHVL
Tag :
His-Tag
Expression System :
E.coli
Product Type Name :
Recombinant Protein
UniProt No. :
P0AD61
Internal Search Terms :
pykF, pyruvate kinase I, ECK1672, JW1666,ATGP1503-10ug,ATGP1503-20ug,ATGP1503-50ug,ATGP1503-100ug,ATGP1503-250ug,ATGP1503-500ug,ATGP1503-1mg,ATGP1503-10,ATGP1503-20,ATGP1503-50,ATGP1503-100,ATGP1503-250,ATGP1503-500,ATGP1503-1
more info or order :
NKMAX product webpage
company information
NKMAX
SNUH Health Care Innovation Park 6F
172 Dolma-ro, Bundang-gu
Seongnam-si, Gyeonggi-do
https://www.nkmaxbio.com
82-31-8017-8114
headquarters: South Korea
NKMAX Co. Ltd. is a biotech company that manufactures and supplies a unique line of quality proteins and antibodies worldwide to both researchers and retailers.