Recombinant E.coli mdh protein | NKMAX ATGP1487 product information

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant E.coli mdh protein

catalog :

ATGP1487

quantity :

20 ug

price :

80 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant E.coli mdh protein

Domain :

1-312aa

Catalog number (SKU) :

ATGP1487

NCBI :

NP_417703

Description :

Malate dehydrogenase (mdh) belongs to the LDH/MDH superfamily and MDH type 1 family. This enzyme catalyzes the conversion of malate into oxaloacetate (using NAD+) and vice versa (this is a reversible reaction). Malate dehydrogenase is also involved in gluconeogenesis, the synthesis of glucose from smaller molecules. Recombinant E. coli mdh protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Alternative Names :

malate dehydrogenase, ECK3225, JW3205

Concentration :

1mg/ml (determined by Bradford assay)

Formulation :

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 50mM NaCl

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Goward CR., et al. (1994). Protein Sci. 3 (10): 1883-8.;; McAlister-Henn L., et al. (1988). Trends Biochem. Sci. 13 (5): 178-81.

Purity :

> 95% by SDS-PAGE

Bioactivity Comment Only :

Specific activity is > 1000unit/mg, and is defined as the amount of enzyme that cleaves 1umole of oxalacetate and beta-NADH to L-malate and beta-NAD per minute at pH8.0 at 37C.

Applications :

SDS-PAGE, Enzyme Activity

Antigen Species :

E.coli

Gene Category :

Metabolism

Molecular Weight :

34.9 kDa (336aa) confirmed by MALDI-TOF

Bioactivity Comment and Pics :

Specific activity is > 1000 units/mg, and is defined as the amount of enzyme that cleaves 1umole of oxalacetate and beta-NADH to L-malate and beta-NAD per minute at pH8.0 at 37C.

Amino Acid Sequence :

MGSSHHHHHH SSGLVPRGSH MGSHMKVAVL GAAGGIGQAL ALLLKTQLPS GSELSLYDIA PVTPGVAVDL SHIPTAVKIK GFSGEDATPA LEGADVVLIS AGVARKPGMD RSDLFNVNAG IVKNLVQQVA KTCPKACIGI ITNPVNTTVA IAAEVLKKAG VYDKNKLFGV TTLDIIRSNT FVAELKGKQP GEVEVPVIGG HSGVTILPLL SQVPGVSFTE QEVADLTKRI QNAGTEVVEA KAGGGSATLS MGQAAARFGL SLVRALQGEQ GVVECAYVEG DGQYARFFSQ PLLLGKNGVE ERKSIGTLSA FEQNALEGML DTLKKDIALG EEFVNK

Tag :

His-Tag

Expression System :

E.coli

Product Type Name :

Recombinant Protein

UniProt No. :

P61889

Internal Search Terms :

mdh, malate dehydrogenase, ECK3225, JW3205,ATGP1487-10ug,ATGP1487-20ug,ATGP1487-50ug,ATGP1487-100ug,ATGP1487-250ug,ATGP1487-500ug,ATGP1487-1mg,ATGP1487-10,ATGP1487-20,ATGP1487-50,ATGP1487-100,ATGP1487-250,ATGP1487-500,ATGP1487-1

more info or order :

NKMAX product webpage