protein

Recombinant Influenza A H1N1 Hemagglutinin/HA1 protein

ATGP1484

10 ug

105 USD

Recombinant Influenza A H1N1 Hemagglutinin/HA1 protein

18-344aa

ATGP1484

ACV04238.1

HA1 (hemaggulutinin1) belongs to the influenza viruses hemagglutinin family. Influenza hemagglutinin (HA) or haemagglutinin is a type of hemagglutinin found on the surface of the influenza viruses. It is an antigenic glycoprotein. It is responsible for binding the virus to the cell that is being infected. HA protein has two functions. Firstly, it allows the recognition of target vertebrate cells, accomplished through the binding of these cells' sialic acid-containing receptors. Secondly, once bound it facilitates the entry of the viral genome into the target cells by causing the fusion of host endosomal membrane with the viral membrane. Recombinant Influenza A virus (A/ New York/3571/2009 (H1N1) ) HA1 protein, fused to His-tag at C-terminus, was expressed in insect cell using baculovirus expression system and purified by using conventional chromatography.

hemagglutinin, Influenza A virus (A/New York/3571/2009 H1N1) hemagglutinin, HA

1mg/ml (determined by Bradford assay)

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 10% glycerol

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Russell RJ, et al. (2008) Proc. Natl. Acad. Sci. u.S.A. 105 (46): 17736-41.;; White JM, et al. (1997) Structural Biology of Viruses. Oxford university Press. pp. 80-104.

> 90% by SDS-PAGE

SDS-PAGE

Human, Influenza A-H1N1, Influenza A-H1N1 (human), Influenza A

Other

37.8 kDa (339aa)

< 1 EU per 1ug of protein (determined by LAL method)

MDTLCIG YHANNSTDTV DTVLEKNVTV THSVNLLEDK HNGKLCKLRG VAPLHLGKCN IAGWILGNPE CESLSTASSW SYIVETSSSD NGTCYPGDFI DYEELREQLS SVSSFERFEI FPKTSSWPNH DSNKGVTAAC PHAGAKSFYK NLIWLVKKGN SYPKLSKSYI NDKGKEVLVL WGIHHPSTSA DQQSLYQNAD AYVFVGSSRY SKKFKPEIAI RPKVRDQEGR MNYYWTLVEP GDKITFEATG NLVVPRYAFA MERNAGSGII ISDTPVHDCN TTCQTPKGAI NTSLPFQNIH PITIGKCPKY VKSTKLRLAT GLRNVPSIQS R

His-Tag

Baculovirus

Recombinant Protein

C7RYS4

Hemagglutinin,ATGP1484-10ug,ATGP1484-20ug,ATGP1484-50ug,ATGP1484-100ug,ATGP1484-250ug,ATGP1484-500ug,ATGP1484-1mg,ATGP1484-10,ATGP1484-20,ATGP1484-50,ATGP1484-100,ATGP1484-250,ATGP1484-500,ATGP1484-1

Yang E, et al. Protection of mice against pandemic H1N1 influenza virus challenge after immunization with baculovirus-expressed stabilizing peptide fusion hemagglutinin protein. J Microbiol Biotechnol. 2015 Feb;25(2):280-7.{https://pubmed.ncbi.nlm.nih.gov/25394603/}