protein

Recombinant M. tuberculosis ESAT6 protein

ATGP1480

10 ug

105 USD

Recombinant M. tuberculosis ESAT6 protein

1-95aa

ATGP1480

NP_338543.1

ESAT6, the 6 kDa early secretory antigenic target of Mycobacterium tuberculosis, is a secretory protein and potent T cell antigen. ESAT6 has been shown to directly bind to the TLR2 receptor, inhibiting downstream signal transduction. Recently it was shown that ESAT6 is very potential as diagnostic for differentiation between the mycobacterial infection and BCG vaccination. Recombinant Mycobacterium tuberculosis CDC1551 ESAT6 protein, fused to His-tag at C-terminus, was expressed in Baculovirus and purified by using conventional chromatography techniques.

Early secretory antigenic target 6 kDa,esxA, esaT6

0.5mg/ml (determined by BCA assay)

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing, 10% glycerol

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Pathak SK., et al. (2007) Natlmmunol. 8(6):610-8.;; Matveeva NA., et al. (2011) Tsitol Genet. 45(1):11-7.

> 90% by SDS-PAGE

SDS-PAGE

Mycobacterium tuberculosis

Other

11 kDa (104aa)

< 1 EU per 1ug of protein (determined by LAL method)

MTEQQWN FAGIEAAASA IQGNVTSIHS LLDEGKQSLT KLAAAWGGSG SEAYQGVQQK WDATATELNN ALQNLARTIS EAGQAMASTE GNVTGMFA

His-Tag

Baculovirus

Recombinant Protein

P9WNK6

ESAT6,ATGP1480-10ug,ATGP1480-20ug,ATGP1480-50ug,ATGP1480-100ug,ATGP1480-250ug,ATGP1480-500ug,ATGP1480-1mg,ATGP1480-10,ATGP1480-20,ATGP1480-50,ATGP1480-100,ATGP1480-250,ATGP1480-500,ATGP1480-1, Early secretory antigenic target, 6 kDa

Satake Y, et al. Type-1 polarised dendritic cells are a potent immunogen against Mycobacterium tuberculosis. (Int J Tuberc Lung Dis. 2017){https://pubmed.ncbi.nlm.nih.gov/28399967/};;Ouyang W, et al. Enabling electrical biomolecular detection in high ionic concentrations and enhancement of the detection limit thereof by coupling a nanofluidic crystal with reconfigurable ion concentration polarization. (Lab Chip. 2017){https://pubmed.ncbi.nlm.nih.gov/28983543/}