Recombinant human Glutathione S-transferase mu 3/GSTM3 protein

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant human Glutathione S-transferase mu 3/GSTM3 protein

catalog :

ATGP1463

quantity :

20 ug

price :

80 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant human Glutathione S-transferase mu 3/GSTM3 protein

Domain :

1-225aa

Catalog number (SKU) :

ATGP1463

NCBI :

NP_000840

Description :

Glutathione S-transferase mu 3, also known as GSTM3, is member of the glutathione s-transferase (GST) family of proteins. There are eight families of GST proteins, namely alpha, kappa, mu, omega, pi, sigma, theta and zeta, each of which is composed of proteins that have a variety of functions throughout the cell. The mu class of enzymes functions in the detoxification of electrophilic compounds, including carcinogens, therapeutic drugs, environmental toxins and products of oxidative stress, by conjugation with glutathione. Recombinant human GSTM3 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Alternative Names :

Glutathione S-transferase mu 3, GST5, GSTB, GSTM3-3, GTM3

Concentration :

1mg/ml (determined by Bradford assay)

Formulation :

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 0.1M NaCl

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Boqaards JJ., et al. (1992) Biochem J. 289(2):383-8.;; Rozell B., et al. (1992) Xenobiotica. 23(8):835-49.

Purity :

> 95% by SDS-PAGE

Bioactivity Comment Only :

Specific activity is > 15,000pmol/min/ug, and is defined as the amount of enzyme that conjugate 1.0 u mole of 1-chloro-2,4-dinitrobenzene (CDNB) with reduced glutathione per minute at pH 6.5 at 25C.

Applications :

SDS-PAGE, Enzyme Activity

Antigen Species :

Human

Gene Category :

Metabolism

Molecular Weight :

29.1 kDa (249aa) confirmed by MALDI-TOF

Bioactivity Comment and Pics :

Specific activity is > 15,000 pmol/min/ug, and is defined as the amount of enzyme that conjugate 1.0 u mole of 1-chloro-2,4-dinitrobenzene (CDNB) with reduced glutathione per minute at pH 6.5 at 25C.

Amino Acid Sequence :

MGSSHHHHHH SSGLVPRGSH MGSHMSCESS MVLGYWDIRG LAHAIRLLLE FTDTSYEEKR YTCGEAPDYD RSQWLDVKFK LDLDFPNLPY LLDGKNKITQ SNAILRYIAR KHNMCGETEE EKIRVDIIEN QVMDFRTQLI RLCYSSDHEK LKPQYLEELP GQLKQFSMFL GKFSWFAGEK LTFVDFLTYD ILDQNRIFDP KCLDEFPNLK AFMCRFEALE KIAAYLQSDQ FCKMPINNKM AQWGNKPVC

Tag :

His-Tag

Expression System :

E.coli

Product Type Name :

Recombinant Protein

UniProt No. :

P21266

Internal Search Terms :

GSTM3, Glutathione S-transferase mu 3, GST5, GSTB, GSTM3-3, GTM3,ATGP1463-10ug,ATGP1463-20ug,ATGP1463-50ug,ATGP1463-100ug,ATGP1463-250ug,ATGP1463-500ug,ATGP1463-1mg,ATGP1463-10,ATGP1463-20,ATGP1463-50,ATGP1463-100,ATGP1463-250,ATGP1463-500,ATGP1463-1

more info or order :

NKMAX product webpage