Recombinant human ALAD protein | NKMAX ATGP1444 product information

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant human ALAD protein

catalog :

ATGP1444

quantity :

10 ug

price :

80 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant human ALAD protein

Domain :

1-330aa

Catalog number (SKU) :

ATGP1444

NCBI :

NP_000022

Description :

The ALAD enzyme is composed of 8 identical subunits and catalyzes the condensation of 2 molecules of delta-aminolevulinate to form porphobilinogen (a precursor of heme, cytochromes and other hemoproteins). ALAD catalyzes the second step in the porphyrin and heme biosynthetic pathway; zinc is essential for enzymatic activity. ALAD enzymatic activity is inhibited by lead and a defect in the ALAD structural gene can cause increased sensitivity to lead poisoning and acute hepatic porphyria. Recombinant human ALAD protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Alternative Names :

Delta-aminolevulinic acid dehydratase, ALADH, PBGS

Concentration :

1mg/ml (determined by Bradford assay)

Formulation :

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 10% glycerol 0.1M NaCl

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Li,C. et al. (2011) Toxicol. Lett. 203 (1), 48-53;; van Bemmel et al. (2011) Epidemiology 22 (2), 273-278

Purity :

> 85% by SDS-PAGE

Applications :

SDS-PAGE

Antigen Species :

Human

Gene Category :

Enzymes & Proteases

Molecular Weight :

38.8 kDa (354aa) confirmed by MALDI-TOF

Amino Acid Sequence :

MQPQSV LHSGYFHPLL RAWQTATTTL NASNLIYPIF VTDVPDDIQP ITSLPGVARY GVKRLEEMLR PLVEEGLRCV LIFGVPSRVP KDERGSAADS EESPAIEAIH LLRKTFPNLL VACDVCLCPY TSHGHCGLLS ENGAFRAEES RQRLAEVALA YAKAGCQVVA PSDMMDGRVE AIKEALMAHG LGNRVSVMSY SAKFASCFYG PFRDAAKSSP AFGDRRCYQL PPGARGLALR AVDRDVREGA DMLMVKPGMP YLDIVREVKD KHPDLPLAVY HVSGEFAMLW HGAQAGAFDL KAAVLEAMTA FRRAGADIII TYYTPQLLQW LKEE

Tag :

His-Tag

Expression System :

E.coli

Product Type Name :

Recombinant Protein

UniProt No. :

P13716

Internal Search Terms :

ALAD, Delta-aminolevulinic acid dehydratase, ALADH, PBGS,ATGP1444-10ug,ATGP1444-20ug,ATGP1444-50ug,ATGP1444-100ug,ATGP1444-250ug,ATGP1444-500ug,ATGP1444-1mg,ATGP1444-10,ATGP1444-20,ATGP1444-50,ATGP1444-100,ATGP1444-250,ATGP1444-500,ATGP1444-1

more info or order :

NKMAX product webpage