Recombinant human UBA3 protein | NKMAX ATGP1425 product information

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant human UBA3 protein

catalog :

ATGP1425

quantity :

20 ug

price :

80 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant human UBA3 protein

Domain :

1-463aa

Catalog number (SKU) :

ATGP1425

NCBI :

AAH22853

Description :

uBA3, also known as NEDD8-activating enzyme E1 catalytic subunit, is the catalytic subunit of the dimeric uBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-uBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of uBE2M. Recombinant human uBA3 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Alternative Names :

NEDD8-activating enzyme E1 catalytic subunit, uBE1C

Concentration :

1mg/ml (determined by Bradford assay)

Formulation :

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 1mM DTT

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Gong L., et al. (1999) J. Biol. Chem. 274:12036-12042;; Bohnsack R.N., et al. (2003) J. Biol. Chem. 278:26823-26830;;

Purity :

> 90% by SDS-PAGE

Applications :

SDS-PAGE

Antigen Species :

Human

Gene Category :

Ubiquitination

Molecular Weight :

54.4 kDa (487aa)

Amino Acid Sequence :

MGSSHHHHHH SSGLVPRGSH MGSHMADGEE PERKRRRIEE LLAEKMAVDG GCGDTGDWEG RWNHVKKFLE RSGPFTHPDF EPSTESLQFL LDTCKVLVIG AGGLGCELLK NLALSGFRQI HVIDMDTIDV SNLNRQFLFR PKDIGRPKAE VAAEFLNDRV PNCNVVPHFN KIQDFNDTFY RQFHIIVCGL DSIIARRWIN GMLISLLNYE DGVLDPSSIV PLIDGGTEGF KGNARVILPG MTACIECTLE LYPPQVNFPM CTIASMPRLP EHCIEYVRML QWPKEQPFGE GVPLDGDDPE HIQWIFQKSL ERASQYNIRG VTYRLTQGVV KRIIPAVAST NAVIAAVCAT EVFKIATSAY IPLNNYLVFN DVDGLYTYTF EAERKENCPA CSQLPQNIQF SPSAKLQEVL DYLTNSASLQ MKSPAITATL EGKNRTLYLQ SVTSIEERTR PNLSKTLKEL GLVDGQELAV ADVTTPQTVL FKLHFTS

Tag :

His-Tag

Expression System :

E.coli

Product Type Name :

Recombinant Protein

UniProt No. :

Q8TBC4

Internal Search Terms :

NEDD8 E1, APPBP1, UBA3, NEDD8-activating enzyme E1 catalytic subunit, uBE1C,ATGP1425-10ug,ATGP1425-20ug,ATGP1425-50ug,ATGP1425-100ug,ATGP1425-250ug,ATGP1425-500ug,ATGP1425-1mg,ATGP1425-10,ATGP1425-20,ATGP1425-50,ATGP1425-100,ATGP1425-250,ATGP1425-500,ATGP1425-1

more info or order :

NKMAX product webpage