Recombinant e.coli gor protein | NKMAX ATGP1423 product information

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant e.coli gor protein

catalog :

ATGP1423

quantity :

20 ug

price :

80 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant e.coli gor protein

Domain :

1-450aa

Catalog number (SKU) :

ATGP1423

NCBI :

NP_417957

Description :

gor, also known as Glutathione reductase, belongs to the class-I pyridine nucleotide disulfide oxidoreductase family. The main function of the protein is to maintain high levels of reduced glutathione in the cytosol. With the concomitant oxidation of NADPH, Glutathione reductase transforms oxidized glutathione to the reduced form. The active site of the protein is a redox-active disulfide bond. Recombinant E. coli gor protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Alternative Names :

Glutathione oxidoreductase, ECK3485, gorA, JW3467

Concentration :

1mg/ml (determined by Bradford assay)

Formulation :

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 0.1M NaCl,1mM DTT

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Staal G.E. et al. (1969) Biochim. Biophys. Acta 185: 63-69.;; Stoll V.S. et al. (1997) Biochemistry 36: 6437-6447.

Purity :

> 90% by SDS-PAGE

Bioactivity Comment Only :

Specific activity is > 60unit/mg. The unit definition for glutathione reductase activity may be expressed in terms of the oxidation of NADPH or the reduction of GSSG since their molar ratio is 1:1. One unit of glutathione reductase oxidizes 1 umol of NADPH per minute at 37C, pH 7.5.

Applications :

Enzyme Activity,SDS-PAGE

Antigen Species :

E.coli

Gene Category :

Enzymes & Proteases

Molecular Weight :

51.2 kDa (473aa) confirmed by MALDI-TOF

Bioactivity Comment and Pics :

Specific activity is > 60 units/mg. The unit definition for glutathione reductase activity may be expressed in terms of the oxidation of NADPH or the reduction of GSSG since their molar ratio is 1:1. One unit of glutathione reductase oxidizes 1 umol of NADPH per minute at 37C, pH 7.5.

Amino Acid Sequence :

MGSSHHHHHH SSGLVPRGSH MGSMTKHYDY IAIGGGSGGI ASINRAAMYG QKCALIEAKE LGGTCVNVGC VPKKVMWHAA QIREAIHMYG PDYGFDTTIN KFNWETLIAS RTAYIDRIHT SYENVLGKNN VDVIKGFARF VDAKTLEVNG ETITADHILI ATGGRPSHPD IPGVEYGIDS DGFFALPALP ERVAVVGAGY IAVELAGVIN GLGAKTHLFV RKHAPLRSFD PMISETLVEV MNAEGPQLHT NAIPKAVVKN TDGSLTLELE DGRSETVDCL IWAIGREPAN DNINLEAAGV KTNEKGYIVV DKYQNTNIEG IYAVGDNTGA VELTPVAVAA GRRLSERLFN NKPDEHLDYS NIPTVVFSHP PIGTVGLTEP QAREQYGDDQ VKVYKSSFTA MYTAVTTHRQ PCRMKLVCVG SEEKIVGIHG IGFGMDEMLQ GFAVALKMGA TKKDFDNTVA IHPTAAEEFV TMR

Tag :

His-Tag

Expression System :

E.coli

Product Type Name :

Recombinant Protein

UniProt No. :

P06715

Internal Search Terms :

gor, Glutathione oxidoreductase, ECK3485, gorA, JW3467,ATGP1423-10ug,ATGP1423-20ug,ATGP1423-50ug,ATGP1423-100ug,ATGP1423-250ug,ATGP1423-500ug,ATGP1423-1mg,ATGP1423-10,ATGP1423-20,ATGP1423-50,ATGP1423-100,ATGP1423-250,ATGP1423-500,ATGP1423-1

more info or order :

NKMAX product webpage