protein

Recombinant human TARS1 protein

ATGP1417

10 ug

105 USD

Recombinant human TARS1 protein

1-723aa

ATGP1417

NP_689508

TARS (Threonyl-tRNA synthetase, cytoplasmic) belongs to the class-II aminoacyl-tRNA synthetase family. This protein has its main role in tRNA aminoacylation. The N-terminal domain of the enzyme is responsible for the competition with the ribosome whilst the catalytic and the C-terminal domain are involved in binding the two anticodon arm-like structures in the operator. Recombinant human TARS protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

threonyl-tRNA synthetase cytoplasmic, threonyl-tRNA synthetase, cytoplasmic, ThrRS

0.5mg/ml (determined by Bradford assay)

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 2mM DTT, 20% glycerol, 150mM NaCl

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Freist W, et al. (1995). Biol. Chem. Hoppe-Seyler 376 (4): 213-24.;; Pan F, et al. (1982). Int. J. Pept. Protein Res. 20 (2): 159-66.

> 85% by SDS-PAGE

SDS-PAGE

Human

Metabolism

85.6 kDa (743aa)

MFEEKASSPS GKMGGEEKPI GAGEEKQKEG GKKKNKEGSG DGGRAELNPW PEYIYTRLEM YNILKAEHDS ILAEKAEKDS KPIKVTLPDG KQVDAESWKT TPYQIACGIS QGLADNTVIA KVNNVVWDLD RPLEEDCTLE LLKFEDEEAQ AVYWHSSAHI MGEAMERVYG GCLCYGPPIE NGFYYDMYLE EGGVSSNDFS SLEALCKKII KEKQAFERLE VKKETLLAMF KYNKFKCRIL NEKVNTPTTT VYRCGPLIDL CRGPHVRHTG KIKALKIHKN SSTYWEGKAD METLQRIYGI SFPDPKMLKE WEKFQEEAKN RDHRKIGRDQ ELYFFHELSP GSCFFLPKGA YIYNALIEFI RSEYRKRGFQ EVVTPNIFNS RLWMTSGHWQ HYSENMFSFE VEKELFALKP MNCPGHCLMF DHRPRSWREL PLRLADFGVL HRNELSGALT GLTRVRRFQQ DDAHIFCAME QIEDEIKGCL DFLRTVYSVF GFSFKLNLST RPEKFLGDIE VWDQAEKQLE NSLNEFGEKW ELNSGDGAFY GPKIDIQIKD AIGRYHQCAT IQLDFQLPIR FNLTYVSHDG DDKKRPVIVH RAILGSVERM IAILTENYGG KWPFWLSPRQ VMVVPVGPTC DEYAQKVRQQ FHDAKFMADI DLDPGCTLNK KIRNAQLAQY NFILVVGEKE KISGTVNIRT RDNKVHGERT ISETIERLQQ LKEFRSKQAE EEF

His-Tag

E.coli

Recombinant Protein

P26639

TDP43, TARDBP, threonyl-tRNA synthetase cytoplasmic, threonyl-tRNA synthetase, cytoplasmic, ThrRS,ATGP1417-10ug,ATGP1417-20ug,ATGP1417-50ug,ATGP1417-100ug,ATGP1417-250ug,ATGP1417-500ug,ATGP1417-1mg,ATGP1417-10,ATGP1417-20,ATGP1417-50,ATGP1417-100,ATGP1417-250,ATGP1417-500,ATGP1417-1

Wang W, et al. The inhibition of TDP-43 mitochondrial localization blocks its neuronal toxicity. (Nat Med. 2016){https://pubmed.ncbi.nlm.nih.gov/27348499/}