Recombinant human DUSP10 protein | NKMAX ATGP1392 product information

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant human DUSP10 protein

catalog :

ATGP1392

quantity :

10 ug

price :

57 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant human DUSP10 protein

Domain :

149-482aa

Catalog number (SKU) :

ATGP1392

NCBI :

NP_009138

Description :

DuSP10 (Dual specificity protein phosphatase 10) belongs to the protein-tyrosine phosphatase family. Dual specificity protein phosphatases (DuSPs) inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the MAPK superfamily (MAPK/ERK, SAPK/JNK, p38), which is associated with cellular proliferation and differentiation. DuSP10 has been shown to interact with MAPK14 and MAPK8. When expressed in mammalian cells, DuSP10 blocks the enzymatic activation of MAP kinases with the selectivity p38 approximately JNK/SAPK >> ERK. Recombinant human DuSP10 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Alternative Names :

Dual specificity protein phosphatase 10, MKP-5, MKP5, Mitogen-activated protein kinase phosphatase 5, MAP kinase phosphatase 5

Concentration :

0.25mg/ml (determined by Bradford assay)

Formulation :

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 2mM DTT, 50% glycerol, 200mM NaCl

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Theodosiou A, et al. (1999) Oncogene. 18(50):6981-8.;; Jeong DG, et al. (2006) J Mol Biol. 360(5):946-55.

Purity :

> 90% by SDS-PAGE

Bioactivity Comment Only :

Specific activity is > 600unit/mg, and is defined as the amount of enzyme that hydrolyze 1.0nmole of p-nitrophenyl phosphate (pNPP) per minute at pH 7.5 at 37C.

Applications :

SDS-PAGE, Enzyme Activity

Antigen Species :

Human

Gene Category :

Enzymes & Proteases

Molecular Weight :

40.4 kDa (359aa) confirmed by MALDI-TOF

Bioactivity Comment and Pics :

Specific activity is > 600 units/mg, and is defined as the amount of enzyme that hydrolyze 1.0 nmole of p-nitrophenyl phosphate (pNPP) per minute at pH 7.5 at 37C.

Amino Acid Sequence :

IIYPN DLAKKMTKCS KSHLPSQGPV IIDCRPFMEY NKSHIQGAVH INCADKISRR RLQQGKITVL DLISCREGKD SFKRIFSKEI IVYDENTNEP SRVMPSQPLH IVLESLKREG KEPLVLKGGL SSFKQNHENL CDNSLQLQEC REVGGGASAA SSLLPQPIPT TPDIENAELT PILPFLFLGN EQDAQDLDTM QRLNIGYVIN VTTHLPLYHY EKGLFNYKRL PATDSNKQNL RQYFEEAFEF IEEAHQCGKG LLIHCQAGVS RSATIVIAYL MKHTRMTMTD AYKFVKGKRP IISPNLNFMG QLLEFEEDLN NGVTPRILTP KLMGVETVV

Tag :

His-Tag

Expression System :

E.coli

Product Type Name :

Recombinant Protein

UniProt No. :

Q9Y6W6

Internal Search Terms :

DUSP10, Dual specificity protein phosphatase 10, MKP-5, MKP5,ATGP1392-10ug,ATGP1392-20ug,ATGP1392-50ug,ATGP1392-100ug,ATGP1392-250ug,ATGP1392-500ug,ATGP1392-1mg,ATGP1392-10,ATGP1392-20,ATGP1392-50,ATGP1392-100,ATGP1392-250,ATGP1392-500,ATGP1392-1

more info or order :

NKMAX product webpage