Recombinant human CHMP4A protein | NKMAX ATGP1376 product information

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant human CHMP4A protein

catalog :

ATGP1376

quantity :

10 ug

price :

240 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant human CHMP4A protein

Domain :

1-265aa

Catalog number (SKU) :

ATGP1376

NCBI :

NP_054888

Description :

CHMP4A (Chromatin-modifying protein 4a) belongs to the SNF7 family and functions as chromatin-modifying proteins. It probable core component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. Also during HIV-1 infection, the virus uses the ESCRT-III complex to mediate budding and exocytosis of viral proteins via the association of CHMP4 and a protein recruited by HIV-1 p6, which is present in viral Gag assembly and budding. CHMP4A expressed at higher level in heart, kidney, liver and skeletal muscle. Recombinant human CHMP4A protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Alternative Names :

Charged multivesicular body protein 4A, Chromatin-modifying protein 4a, CHMP4a, SNF7 homolog associated with Alix-2, SNF7-1, hSnf-1, Vacuolar protein sorting-associated protein 32-1, Vps32-1, hVps32-1, HSPC134, VPS32A

Concentration :

0.25mg/ml (determined by Bradford assay)

Formulation :

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 2mM DTT, 50% glycerol, 200mM NaCl, 0.1mM PMSF, 1mM EDTA

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Katoh K., et al. (2004) Arch. Biochem. Biophys. 421:159-165;; Strack B., et al (2003) Cell 114:689-699

Purity :

> 85% by SDS-PAGE

Applications :

SDS-PAGE

Antigen Species :

Human

Gene Category :

Metabolism

Molecular Weight :

31.9kDa (285aa) confirmed by MALDI-TOF

Amino Acid Sequence :

MSRRRPEDGL GKAGPCVMRH HPPRSKAEVW RTLRGGGGRG ELAMSGLGRL FGKGKKEKGP TPEEAIQKLK ETEKILIKKQ EFLEQKIQQE LQTAKKYGTK NKRAALQALR RKKRFEQQLA QTDGTLSTLE FQREAIENAT TNAEVLRTME LAAQSMKKAY QDMDIDKVDE LMTDITEQQE VAQQISDAIS RPMGFGDDVD EDELLEELEE LEQEELAQEL LNVGDKEEEP SVKLPSVPST HLPAGPAPKV DEDEEALKQL AEWVS

Tag :

His-Tag

Expression System :

E.coli

Product Type Name :

Recombinant Protein

UniProt No. :

Q9BY43

Internal Search Terms :

CHMP4A, chromatin modifying protein 4A, CHMP4, CHMP4B, HSPC134, SHAX2, SNF7, SNF7-1, VPS32-1, VPS32A,ATGP1376-10ug,ATGP1376-20ug,ATGP1376-50ug,ATGP1376-100ug,ATGP1376-250ug,ATGP1376-500ug,ATGP1376-1mg,ATGP1376-10,ATGP1376-20,ATGP1376-50,ATGP1376-100,ATGP1376-250,ATGP1376-500,ATGP1376-1

more info or order :

NKMAX product webpage