protein

Recombinant human HMGCL protein

ATGP1345

20 ug

80 USD

Recombinant human HMGCL protein

28-325aa

ATGP1345

NP_000182

HMGCL, also known as hydroxymethylglutaryl-CoA lyase, is a mitochondrial matrix protein that belongs to the HMG-CoA lyase family. It exists as a homodimer and participates in leucine catabolism and ketogenesis, the hepatic synthesis of ketone bodies that, during fasting, provide a major source of energy for heart, brain and kidney. More specifically, it catalyzes the final step of these processes, the cleavage of 3-hydroxy-3-methylglutaryl-CoA to acetoacetic acid and acetyl-CoA. Recombinant human HMGCL protein, fused to His-tag at N-terminus, was expressed in E. coli.

Hydroxymethylglutaryl-CoA lyase, 3-hydroxy-3-methylglutarate-CoA lyase, HL, HMG-CoA lyase, Hydroxymethylglutaryl-CoA lyase mitochondrial

1mg/ml (determined by Bradford assay)

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 0.4M urea

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Cardoso M.L. et al. (2004) Mol. Genet. Metab. 82: 334-338.;; Wang S.P et al. (1996) Genomics 33: 99-104.

> 85% by SDS-PAGE

SDS-PAGE

Human

Cancer

34.2 kDa (323aa)

MGSSHHHHHH SSGLVPRGSH MGSHMTLPKR VKIVEVGPRD GLQNEKNIVS TPVKIKLIDM LSEAGLSVIE TTSFVSPKWV PQMGDHTEVL KGIQKFPGIN YPVLTPNLKG FEAAVAAGAK EVVIFGAASE LFTKKNINCS IEESFQRFDA ILKAAQSANI SVRGYVSCAL GCPYEGKISP AKVAEVTKKF YSMGCYEISL GDTIGVGTPG IMKDMLSAVM QEVPLAALAV HCHDTYGQAL ANTLMALQMG VSVVDSSVAG LGGCPYAQGA SGNLATEDLV YMLEGLGIHT GVNLQKLLEA GNFICQALNR KTSSKVAQAT CKL

His-Tag

E.coli

Recombinant Protein

P35914

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